What is human prostatic acid phosphatase?
What is human prostatic acid phosphatase?
Prostatic acid phosphatase (PAP), also prostatic specific acid phosphatase (PSAP), is an enzyme produced by the prostate. It may be found in increased amounts in men who have prostate cancer or other diseases. The highest levels of acid phosphatase are found in metastasized prostate cancer.
What type of enzyme is acid phosphatase?
lysosomal enzyme
Acid phosphatase is a ubiquitous lysosomal enzyme that hydrolyses organic phosphates at an acid pH. Although the postpuberteral prostatic epithelial cell contains a uniquely high concentration of acid phosphatase, cellular components of bone, spleen, kidney, liver, intestine, and blood also contain this enzyme.
What are the substrates used in the determination of total acid phosphatase?
Acid phosphatase of serum acts on the monophosphate thymolphthalein substrate. The addiction of alkali inhibits the enzymatic action and converts the released thymolphthalein in it blue form that is measured by colorimetry. The final product of reaction consists of a mixture of blue and the substrate color.
What is the normal range of acid phosphatase?
In adults and elderly persons, the normal findings for acid phosphatase are 0.13-0.63 U/L (Roy, Brower, Hayden; 37°C) or 2.2-10.5 U/L (SI units). Normal findings in children are 8.6-12.6 U/mL (30°C), while normal findings in newborns are 10.4-16.4 U/mL (30°C).
Is the acid phosphatase human specific?
Human prostatic acid phosphatase (PAcP; E.C. 3.1. 3.2) is a prostate epithelium-specific differentiation antigen found in large amounts initially in seminal fluid.
How is acid phosphatase made?
The acid phosphatases are lysosomal enzymes that hydrolyse phosphomonoesters at low pH, releasing phosphoric acid. They are produced in a number of tissues, including bone, prostate, platelets and erythrocytes. Five isoenzymes are detectable in plasma.
What is a phosphatase enzyme?
Phosphatases or phosphomonoesterases are the hydrolytic enzymes that cleave the ester bond between the phosphate group and the organic residue of the organic phosphates. Based on the optimum pH for the activity, phosphatases are of two kinds: acid and alkaline.
What is Bessey Lowry Brock method?
In 1946 Bessey, Lowry and Brock [4] used p-nitrophenyl phosphate as substrate for the estimation of alkaline serum phosphatase in 5 of serum. In this method of determination it is merely necessary to make the mixture alkaline after incubation and to measure the color intensity.
What is the chemical reaction catalyzed by wheat germ acid phosphatase in vivo?
Under acid conditions the enzyme catalyzes the hydrolysis of p-nitrophenyl phosphate to inorganic phosphate and p- nitrophenol (Figure Page 4 5-10).
What is the acid phosphatase test used for?
The acid phosphatase (AP) test is a routine assay used to screen casework items for the possible presence of semen. This colour test is carried out on filter paper which is retained after testing.
What is normal value of acid phosphate?
What is prostatic acid phosphatase?
Prostatic acid phosphatase (PAP), also prostatic specific acid phosphatase (PSAP), is an enzyme produced by the prostate. It may be found in increased amounts in men who have prostate cancer or other diseases. The highest levels of acid phosphatase are found in metastasized prostate cancer.
What is tartrate resistant acid phosphatase?
Tartrate-resistant acid phosphatase. The acid phosphatases are lysosomal enzymes that hydrolyse phosphomonoesters at low pH, releasing phosphoric acid. They are produced in a number of tissues, including bone, prostate, platelets and erythrocytes. Five isoenzymes are detectable in plasma.
Is serum PSAP a biomarker for prostate cancer?
Serum marker. PSAP was used to monitor and assess progression of prostate cancer until the introduction of prostate specific antigen (PSA), which has now largely displaced it. Subsequent work, suggested that it has a role in prognosticating intermediate and high-risk prostate cancer, and led to renewed interest in it as a biomarker.
What is psapsap immunohistochemical staining?
PSAP immunohistochemical staining is often used with PSA (staining), by pathologists, to help distinguish poorly differentiated carcinomas.
