What are destination vectors?
What are destination vectors?
Gateway Destination vectors are similar to classical expression vectors containing multiple cloning sites, before the insertion of a gene of interest, using restriction enzyme digestion and ligation. Gateway Destination vectors are commercially available from Invitrogen, EMD (Novagen) and Covalys.
What are the three methods that you can use to create a gateway entry clone?
There are three methods you can use to produce an Entry clone: BP cloning, restriction enzyme and ligase cloning, and Invitrogen TOPO cloning into a Invitrogen Gateway Entry vector, which is the most common method.
What are gateway technologies?
Gateway Technologies is a diversified national software development and IT outsourcing company that gives technological solutions to business enterprises around the India.
Why clone with experience gateway?
Experience Gateway cloning technology. Accurate results —cloning reactions achieve >95% efficiency to deliver the clone you need DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money. This protocol has been published in BioTechniques.
What is Invitrogen Gateway cloning technology?
The Invitrogen Gateway Cloning Technology provides a rapid and highly efficient route to protein expression, functional analysis, and cloning/subcloning of DNA segments.
What are gategateway-compatible destination vectors?
Gateway-compatible destination vectors contain the Gateway recombination sites, allowing rapid, efficient transfer of sequences into a variety of expression systems using site-specific recombination.
What are the selection markers used for Gateway cloning?
The positive (antibiotic) and negative (CcdB) selection markers used for Gateway Cloning can increase cloning efficiency to >99%. All types of DNA fragments may be cloned: PCR fragments, cDNA or Genomic DNA and is available for all kind of organisms from mammals to E. coli.
