What is cyanine dye used for?
What is cyanine dye used for?
Cyanine dyes are used to label proteins, antibodies, peptides, nucleic acid probes, and any kind of other biomolecules to be used in a variety of fluorescence detection techniques (Ünlü et al., 1997; Osterman et al., 2013) [5,6].
What is cyanine color?
The word cyanin is from the English word “cyan”, which conventionally means a shade of blue-green (close to “aqua”) and is derived from the Greek κυάνεος/κυανοῦς kyaneos/kyanous which means a somewhat different color: “dark blue”.
What is the meaning of cyanine?
Definition of cyanine : any of various dyes used especially to sensitize photographic film to light from the green, yellow, red, and infrared regions of the spectrum.
What Cy5 5?
Cy5. 5 NHS Ester is a bright and photostable near-IR dye that is spectrally similar to Alexa Fluor® 680, DyLight® 680, and IRDye® 680. Cy5. 5 NHS Ester is completely water-soluble and often a reagent of choice for labeling antibodies, peptides, and proteins that are sensitive toward organic co-solvents.
What is Cyanine Blue?
Definition of cyanine blue 1a : a strong blue that is greener and deeper than Sèvres and redder and darker than cerulean blue (see cerulean blue sense 1b) — called also Leitch’s blue.
Is Cy5 far red?
Invitrogen Cy5 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines.
What is FAM fluorophore?
FAM is the most commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection equipment. It becomes protonated and has decreased fluorescence below pH 7; it is typically used in the pH range 7.5–8.5. FAM can be attached to 5′ or 3′ end of oligos.
What wavelength is Tritc?
TRITC (Tetramethylrhodamine-isothiocyanate) is a fluorescent compound with an excitation peak at 544 nm and an emission peak at 570 nm.
Does PE and FITC overlap?
In some experiments FITC may be combined with other dyes, for example PE, that emit yellow and orange photons. Fig. FITC spillover into other channels. FITC single stained lymphocytes show spillover into PE and PE-Cy5 detectors.
What is the future of cyanine dyes chemistry?
The developments of cyanine dyes synthesis and their applications in photographic and non-photographic multidisciplinary areas are growing continuously, significantly and rapidly. Certainly, this will make the present and the future of cyanine dyes chemistry effective, fruitful and very bright.
What is the resonance structure of cyanine dyes?
The structure of most cyanine dyes is characterised by the presence of two resonance forms (two mesomeric structures). These two resonance forms have the responsibility for the intensity of the colour of cyanine dyes where they produce a delocalized positive charge over the conjugated structure system of cyanine dyes.
What is trimethine cyanine dyes?
The dye with 3 methine groups (n=1 in Fig. 1) is classified as trimethine cyanine dyes or carbocyanine dyes, e.g. pinacyanol dye (2) and kryptocyanine dye (3), Fig. 2. Fig. 4.
What is pentamethinium cyanine (dicarbocyanine)?
These dyes were confirmed to contain a similar feature; a pentamethinium cyanine (dicarbocyanine) chromophore substituted with two chiral end groups derived from l -α-amino acids. Betanin, which is responsible for the red-violet colour of the red beet, Beta vulgaris, exhibits a visible absorption at 537 nm.
