What is 4C method?
What is 4C method?
(A) The 4C method entails chromatin fixation, restriction digestion and re-ligation to generate hybrid sequences between fragments that were physically proximal during fixation. The DNA is purified, digested with a secondary restriction enzyme and re-ligated under dilute conditions to generate DNA circles.
What is promoter capture Hi-C?
We developed Promoter Capture Hi-C (PCHi-C) to enable the genome-wide detection of distal promoter-interacting regions (PIRs), for all promoters in a single experiment. The aim is to then pull-down promoter sequences and their frequent interaction partners such as enhancers and other potential regulatory elements.
What are Hi-C maps?
The high-throughput chromosome conformation capture (Hi-C) technique has enabled genome-wide mapping of chromatin interactions. However, high-resolution Hi-C data requires costly, deep sequencing; therefore, it has only been achieved for a limited number of cell types.
What is Hi-C genomics?
Hi-C sequencing is high‐throughput chromosome conformation capture technique to analyze spatial genome organization and map higher‐order chromosome folding and topological associated domains. Hi-C allows quantification of interactions between all possible pairs of DNA fragments simultaneously. …
What is circular chromosome conformation capture?
Circular chromosome conformation capture (4C) uncovers extensive networks of epigenetically regulated intra- and interchromosomal interactions Accumulating evidence converges on the possibility that chromosomes interact with each other to regulate transcription in trans.
What is 4C (chromosome capture-on-chip)?
Chromosome conformation capture-on-chip (4C) captures interactions between one locus and all other genomic loci. It involves a second ligation step, to create self-circularized DNA fragments, which are used to perform inverse PCR. Inverse PCR allows the known sequence to be used to amplify the unknown sequence ligated to it.
What is 3C in chromosome analysis?
Chromosome Conformation Capture Analysis (3C) Techniques. Chromatin Conformation Capture (3C) is an important technique used to study chromatin structure, as well as the basis for several other derivative techniques. 3C provides information on 3D chromatin structures that occur in living cells.
Do chromosomes interact with each other to regulate transcription in trans?
Accumulating evidence converges on the possibility that chromosomes interact with each other to regulate transcription in trans. To systematically explore the epigenetic dimension of such interactions, we devised a strategy termed circular chromosome conformation capture (4C). This approach involves …
