What are BHK cells used for?
What are BHK cells used for?
Baby hamster kidney (BHK) cells are currently used for vaccine production as well as production of a few recombinant proteins such as Factor VIIa (NovoSeven®) and FVIII (Kogenate®).
Which medium is used to culture BHK cells?
The medium for BHK cells is αMEM or DMEM (BioWhittaker). FBS makes the cells grow too fast. Infection with Sindbis viral particles will result in premature cell lysis, leading to low yields of protein expression. Cells are grown in a humidified, 37°C, 5% CO2 incubator.
Are BHK cells adherent?
Baby Hamster Kidney fibroblasts (BHK cells) are an adherent cell line used in molecular biology. The cells were derived in 1961 by I. A. Macpherson and M. G. P. Stoker.
What are C6 36 cells?
C6/36 Aedes albopictus cells are a mosquito cell line commonly used to study arbovirus-vector interactions [34]–[36]. Recently, it was demonstrated that WNV-specific siRNAs could not be detected by northern blot hybridization following infection of C6/36 cells, although siRNAs were found in Drosophila S2 cells [29].
How do you grow viruses?
Viruses replicate only within living cells. Some viruses are restricted in the kinds of cells in which they replicate, and a few have not yet been cultivated at all under laboratory conditions. However, most viruses are grown in cultured cells, embryonated hen’s eggs, or laboratory animals.
Who is known as the father of tissue culture?
Gottlieb Haberlandt is known as the father of plant tissue culture.
What is mosquito cell lines?
Although several mosquito cell lines have been established from major mosquito disease vectors such as An. gambiae (malaria) and Ae. aegypti (dengue), further cell lines are needed for native temperate mosquito species that are considered potential vectors of arboviruses.
What is BHK21 medium used for?
Gibco® CD BHK-21 Production Medium is a chemically defined, protein-free cell culture medium. It is designed to support the growth of BHK-21 cells in serum-free suspension culture. This medium is suitable for large-scale manufacturing applications.
What is the best media for BHK cells?
• The medium for BHK cells is αMEM or DMEM (BioWhittaker). • Complete medium for BHK cells is αMEM + 2 mM L-glutamine + 5% fetal bovine serum. FBS does not need to be heat inactivated for use with BHK cells. Note: 10% FBS makes the cells grow too fast.
What is cdcd BHK-21 production medium?
CD BHK-21 Production Medium is a chemically defined, protein-free cell culture medium that does not contain protein hydrolysates or growth factors. This medium is designed for the growth of BHK-21 cells in serum-free suspension culture and it is suitable for large-scale manufacturing applications.
What is the BHK cell line?
BHK Cells The BHK cell line was derived from baby Syrian hamster (Mesocricetus auratus) kidney (Macpherson and Stoker, 1962). • The medium for BHK cells is αMEM or DMEM (BioWhittaker). • Complete medium for BHK cells is αMEM + 2 mM L-glutamine + 5% fetal bovine serum.
