Does optimem have phenol red?

Does optimem have phenol red?

Opti-MEM™ I Reduced Serum Medium, no phenol red.

Does RPMI contain phenol red?

RPMI 1640 Medium, no phenol red.

Does optimem contain serum?

Using Opti-MEM® I Medium Opti-MEM® I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%.

What is l-15?

L-15 Medium (Leibovitz) was originally formulated for use in carbon dioxide (CO2) free systems requiring sodium bicarbonate. L-15 is buffered by the use of free basic amino acids, phosphate buffers and higher levels of galactose and sodium pyruvate to help maintain physiological pH control.

Can I use DMEM instead of optimem?

In my experience Optimem works better but you can also use successfully for transfection serum-free DMEM or RPMI . Few hours after transfection you then replace the transfection medium with DMEM or RPMI supplemented with serum. Serum free medium works fine, not as much as optimem but it works.

Is phenol red acidic or basic?

It is a weak acid with pKa = 8.00 at 20 °C (68 °F). A solution of phenol red is used as a pH indicator, often in cell culture. Its color exhibits a gradual transition from yellow (λmax = 443 nm) to red (λmax = 570 nm) over the pH range 6.8 to 8.2. Above pH 8.2, phenol red turns a bright pink (fuchsia) color.

Is phenol red fluorescence?

Of note, most tissue culture medium contains fluorescent compounds such as for example phenol red. However, phenol red is highly fluorescent when excited at 440 nm, and phenol red free media has to be used for imaging of cyan FPs.

Can I use Dmem instead of Opti-MEM?

Can cells grow in Opti-MEM?

Most cells grown in serum-supplemented media can be transferred to Opti-MEM media with a minimum of 50% reduction in serum. Opti-MEM I Reduced Serum Media is ideal for use during cationic lipid transfections—especially Invitrogen Lipofectamine transfection reagents.

What is Leibovitz l15?

Leibovitz’s L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate. This medium is designed for supporting cell growth in environments without CO2 equilibration.

Is RPMI serum free?

RPMI 1640 has traditionally been used for the serum-free expansion of human lymphoid cells. RPMI 1640 uses a bicarbonate buffering system and differs from most mammalian cell culture media in its typical pH 8 formulation.

Why Opti-MEM is used in transfection?

Opti-MEM is a serum-reduced media used during transfection of genetic molecules into mammalian cells. Similar amounts of knockdown of the target proteins were achieved in DMEM, and cell viability was higher compared with Opti-MEM after transfection.

What is the purpose of phenol red?

Phenol red (also known as phenolsulfonphthalein or PSP) is a pH indicator frequently used in cell biology laboratories. Phenol red, sometimes labelled with a different name, such as “Guardex Solution #2”, is used as a pH indicator in home swimming pool test kits.

What color does phenol red turn in a basic solution?

A solution of phenol red is used as a pH indicator, often in cell culture. Its color exhibits a gradual transition from yellow (λmax = 443 nm) to red (λmax = 570 nm) over the pH range 6.8 to 8.2. Above pH 8.2, phenol red turns a bright pink (fuchsia) color.

What color is phenol red at neutral pH?

yellow at acid pH, bright pink at alkaline pH. Phenol red is red or orange around neutral pH. What is the color of phenol red at an acid pH and at an alkaline pH? Yellow at acid pH (look for this in carbohydrate broths), bright pink at alkaline pH (look for this in urease broth). Phenol red is red or orange around neutral pH.

What is phenol red indicator used for?

Phenol red is used as a pH indicator. The sodium salt of Phenol Red is widely used in culture media to identify changes of neutral to acidic pH values. A Phenol Red solution has: A yellow color at a pH of 6.4 or below.