What is the recognition sequence for SmaI?

What is the recognition sequence for SmaI?

The SmaI and XmaI endonucleases are imperfect isoschizomers that recognize the sequence CCCGGG.

Which two enzymes are examples of isoschizomers?

Isoschizomers are pairs of restriction enzymes specific to the same recognition sequence. For example, SphI (CGTAC/G) and BbuI (CGTAC/G) are isoschizomers of each other.

Does Ecori have isoschizomers?

Isoschizomers | Single Letter Code | Pronunciation: Time-Saver™ qualified for digestion in 5-15 minutes. High Fidelity (HF®) version available (NEB #R3101) supplied with rCutSmart™ Buffer.

What sequence is cut by Hind 2?

Hind II recognizes the sequence GTPy/PuAC and generates fragments with blunt ends (1). Compatible ends Hind II generates fragments with blunt ends and is compatible to any other blunt end. Isoschizomers Hind II is an isoschizomer to Hinc II. Hind II is inhibited by 6-methyladenine as indicated (*).

What type of ends does SmaI produce?

SmaI is an isoschizomer of XmaI. SmaI produces blunt-ended fragments whereas XmaI produces a 5´ extension.

What do you mean by recognition sequence?

A recognition sequence is a DNA sequence to which a structural motif of a DNA-binding domain exhibits binding specificity. A recognition sequence is different from a recognition site. A given recognition sequence can occur one or more times, or not at all, on a specific DNA fragment.

What is the difference between Isoschizomers and Neoschizomers?

The key difference between isoschizomers and neoschizomers is that isoschizomers are restriction enzymes that have the same recognition sequence and cleave the DNA at the same positions, while neoschizomers are restriction enzymes that have the same recognition sequence but cleave DNA at different positions.

How is the recognition sequence for ecor1 determined?

EcoRI (pronounced “eco R one”) is a restriction endonuclease enzyme isolated from species E. The nucleic acid recognition sequence where the enzyme cuts is G↓AATTC, which has a palindromic, complementary sequence of CTTAA↓G.

What is the recognition sequence of HindIII?

Under the standard reaction conditions, the restriction endonuclease HindIII cleaves double-stranded DNA, within the recognition sequence–A/AGCTT–at the position indicated by the arrow. In the presence of dimethyl sulfoxide the substrate specificity of this enzyme is reduced and cleavages occur at additional sites.