Where is PV92 located?
Where is PV92 located?
chromosome 16
1997 ). One of these polymorphic Alu insertions is the PV92 Alu site that is located in chromosome 16, and it has been proved to be human-specific (Batzer et al. 1994 ).
Why is chelate necessary?
Why is it necessary to chelate the metal ions from solution during the boiling/lysis step at 100 degrees C? You must use a chelating agent because it grabs metal ions out of the solution and traps them, and they are cofactors in enzymatic reactions.
What does the Alu gene code for?
Alu elements are responsible for regulation of tissue-specific genes. They are also involved in the transcription of nearby genes and can sometimes change the way a gene is expressed. Alu elements are retrotransposons and look like DNA copies made from RNA polymerase III-encoded RNAs.
How many primers are needed for a successful PCR reaction?
Two primers
Two primers are used in each PCR reaction, and they are designed so that they flank the target region (region that should be copied).
What is the purpose of Alu elements?
How do Alu elements replicate?
Alu repeats are a family of short interspersed elements (SINEs) that replicate via LINE-mediated reverse transcription of an RNA polymerase III transcript (Rogers 1983; Mathias et al. 1991; Dewannieux et al. 2003). Each Alu element is roughly 280 bp long, followed by a poly-A tail of variable length.
What is the PV92 PCR Informatics kit?
With the PV92 PCR Informatics Kit, your students use real-world forensic techniques to extract DNA from their hair follicles or cheek cells, and then use PCR amplification and electrophoresis to fingerprint their own DNA at a specific genetic locus.
What refill packs are available for the hpv92 PCR kit?
PV92 PCR Kit TS (1662119EDU) and RT (1662139EDU) Refill Packs are available; education use only This product contains electrical or electronic material. Learn more about Bio-Rad’s EU Recycle Program
What is included in the PCR classroom study kit?
PCR classroom study kit, includes positive controls, PCR master mix, primers, extraction matrix, Fast Blast DNA stain, test tubes/holders, curriculum, and more, for 32 students. PV92 PCR Kit TS (1662119EDU) and RT (1662139EDU) Refill Packs are available; education use only
