What is the purpose of tetrathionate broth?
What is the purpose of tetrathionate broth?
Tetrathionate Broth is recommended for the selective enrichment method of isolating Salmonella typhi and other salmonellae from faeces, sewage, etc. Organisms which reduce tetrathionate, such as salmonellae, flourish in the medium whilst many faecal organisms are inhibited1.
How do you make Tetrathionate broth?
- Dissolve 46 g of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minutes to completely dissolve the medium.
- Cool to 45-50°C and add 20 mL of the Iodine-Potassium Iodide Solution to the prepared Tetrathionate Broth Base.
- DO NOT REHEAT AFTER ADDING IODINE SOLUTION.
What is the main selective agent in tetrathionate broth?
Tetrathionate is formed in the medium upon addition of Iodine–Potassium Iodide (I2-KI) solution. It serves as a selective agent, combined with sodium thiosulfate, to suppress coliforms and other microflora.
What is the purpose of using tetrathionate broth in the isolation scheme for salmonella?
What is brilliant green agar?
Brilliant Green Agar is a selective medium used for the isolation Salmonella spp, other than S. Typhi and S. Paratyphi from pathogen materials, stool, urine, environmental samples and food. Brilliant Green Agar is recommended by APHA, FDA and USP.
What is the purpose of using tetrathionate broth in the isolation scheme for Salmonella?
What is GN broth?
GN (Gram Negative) Broth is a selective enrichment medium for the cultivation of gram-negative enteric organisms.
Which is flagellar antigen of Salmonella?
For instance, Salmonella bacteria look alike under the microscope but can be separated into many serotypes based on two structures on their surface: The outermost portion of the bacteria’s surface covering, called the O antigen; and. A slender threadlike structure, called the H antigen, that is part of the flagella.
Which enriched media is used to isolate Salmonella?
The selective enrichment media are considered to be of critical importance in salmonella isolation. During the last 10 years Rappaport-Vassiliadis broth has emerged as a new reliable enrichment medium.
Is Salmonella a typhi?
Salmonella Typhi (S. Typhi) are bacteria that infect the intestinal tract and the blood. The disease is referred to as typhoid fever.
Does E coli grow on brilliant green agar?
These may grow as small red colonies. Yellow to greenish-yellow coloured colonies surrounded by intense yellow-green zones in the agar – Escherichia coli or Klebsiella/Enterobacter group….Result Interpretation on Brilliant Green Agar.
| Organisms | Growth |
|---|---|
| Escherichia coli | Inhibited or no growth; yellowish green |
What is gram negative broth?
Gram Negative Broth is a selective enrichment medium devised by Hajna for cultivation of Gram- negative organisms of the enteric group1, 2. HISTORY/SUMMARY: Hajna (1955) devised a GN (gram negative) broth, which was used in part as a preservative medium and in part as an enrichment for specimens preserved in SP medium.
