Why may you use a frozen sectioning method rather than paraffin sectioning?
Why may you use a frozen sectioning method rather than paraffin sectioning?
Frozen sectioning is the procedure of cutting thin sections of frozen tissue and is conducted in a cryostat. While frozen sections are physically less stable than paraffin, they are especially superior in the preservation of antigenicity and lipid retention.
What are the advantages of frozen sections?
The frozen section biopsy can help ensure that the mass being removed is the intended tissue for removal. It can help ensure that the entire mass and its surrounding borders are removed. It allows for the collection of proper tissue samples for further scientific research.
What are the disadvantages of paraffin embedding?
The fixation and paraffin embedding process can also be time consuming. In studies that are using FFPE tissue samples, one of the disadvantages will be the non-standardized procedures in the preparation of FFPE samples which means that there are no assurances that selected samples are prepared the same way.
Why are frozen sections used for the demonstration of fat in tissue?
The Principle of Frozen Section When the tissue sample goes through rapid freezing, it converts water into ice which acts as an embedding media allowing the tissue to be sectioned. The tissue can become firmer if the temperature of the tissue sample is lowered while increasing the temperature softens the tissue.
How are tissues sectioned?
Sectioning is the process of cutting tissue into thin slices. Tissue is typically embedded with optimal cutting temperature (OCT) or paraffin prior to being sectioned. Frozen tissues are sectioned using Leica cryostats, and paraffin tissues are sectioned using Leica microtomes. …
What is the advantage of cryo sectioning for antibody staining methods?
Compared to paraffin-embedded sections, frozen tissues are thicker, lowering microscopic resolution and the ability to capture tissue morphology in detail. However, cryopreservation is thought to better preserve antigen and antigenicity.
What are the limitations of the frozen section?
Well, what are these limitations? The Manual of Surgical pathology (2) in a section titled” Frozen Sections Are Not Permanent Sections” points to four reasons. These are: sampling error, ice crystal artifacts, lack of special studies, and lack of consultation.
What is advantage and disadvantage of frozen sections cut by microtome?
Frozen section technique shortens the time to preliminary diagnosis significantly from traditional tissue biopsy preparation. Once the specimen is received in the laboratory, pathology results are communicated within a short time.
What is difference between fresh and frozen specimen?
Fixed – how most specimens are sent to pathology and why; Fresh – scenarios where a surgeon might send the tissue without fixation; Frozen – covering intra-operative pathology consults – how this works, and some examples.
What is paraffin embedding technique?
Paraffin embedding is a standard technique used in clinical and research laboratories to create a formalin-fixed, paraffin-embedded (FFPE) block of tissue. Formalin-fixed tissue undergoes tissue processing and then is embedded in paraffin (wax) to create a FFPE block or paraffin block.
What is the difference between Mohs and frozen section?
The frozen sections are stained with histochemical stains that help differentiate normal from cancerous tissue. Then the Mohs surgeon evaluates the tissue under a microscope.
What are the other methods used to freeze tissues in frozen section?
At pathology institutes with higher work loads, solid carbon dioxide, freezing sprays, and cryostat freezing are commonly used as coolants for diagnosing frozen tissue sections, whereas for tissue banking, liquid nitrogen or isopentane cooled with liquid nitrogen is preferred.
