What is difference between IC50 and EC50?
What is difference between IC50 and EC50?
The EC50 is the concentration of a drug that gives half-maximal response. The IC50 is the concentration of an inhibitor where the response (or binding) is reduced by half.
What does EC50 of a drug mean?
half maximal effective concentration
EC. 50. The parameter EC50 abbreviates for ‘half maximal effective concentration’. In a pharmacological context, this can be the concentration of a drug that is necessary to cause half of the maximum possible effect.
Why do we use log concentrations?
Because of this, dose-response curves are usually plotted with the concentration as a logarithmic scale (usually base 10). This makes the relative potency of drugs more easy to compare. The logarithmic graph also gives a more convenient scale at low concentrations where the response is changing rapidly with dose.
What does EC90 mean?
The EC90 was calculated as the concentration of drug that increased the percentage of formazan production in drug treated, virus infected cells to 50% of that produced by drug free uninfected cells on human rhinovirus-2.
Is a high EC50 good?
The lower the EC50, the less the concentration of a drug is required to produce 50% of maximum effect and the higher the potency.
Why do we use EC50?
An EC 50 is a statistical estimate of the concentration of a toxicant in the ambient medium necessary to produce a particular effect in 50% of a very large population under specified conditions. An LC 50 is a special case of the EC 50 in which the effect recorded is death.
What does high EC50 mean?
Potency is related to the effective concentration (EC50). The EC50 indicates how much of a drug is needed to achieve 50% of the maximum response. The more potent a drug, the smaller the EC50 will be. This value is obtained from a dose-response curve.
Is pH scale logarithmic?
The pH scale is logarithmic, essentially meaning the difference in 1 pH unit is a difference of 10 times!
How do I calculate EC90?
If you know the EC50 and Hill slope (H), you can easily compute the EC80 or EC10 or any other value you want. For example, if the Hill slope equals 1, the EC90 equals the EC50 times nine. If H equals 0.5, the curve is shallower and the EC90 equals the EC50 times 81.
What is EC50 in toxicology?
Terminology: EC50(50% Effective Concentration) The median effective concentration (EC50) is the concentration of a substance in an environmental medium expected to produce a certain effect in 50% of test organisms (usually planktonic crustacean Daphnia) in a given population under a defined set of conditions.
Is a low EC50 good?
What does the ec500 measure?
EC500: Waterproof ExStik® II pH/Conductivity Meter Combination flat surface pH electrode with autoranging high accuracy Conductivity cell The EC500 is Conductivity/TDS/Temperature Meter. Measures 5 parameters including Conductivity, TDS, Salinity, pH, and Temperature using one electrode.
What is the difference between EC 50 and Ec 10?
The lower the EC 50, the less the concentration of a drug is required to produce 50% of maximum effect and the higher the potency. The EC 10 and EC 90 concentrations to induce 10% and 90% maximal responses are defined similarly. There is a wide range of EC 50 values of drugs; they are typically anywhere from nM to mM.
When should absolute ec50/ic50 be used?
The absolute EC50/IC50 should only be used if there are at least two assay concentrations whose predicted response is less than 50% and two whose predicted response is greater than 50%. A wide range of typical assay conditions are considered in the development of the guidelines.
Is it possible to define the EC50 without the baseline?
It is impossible to define the EC50 until you first define the baseline and maximum response. Depending on how you have normalized your data, the EC50 may not be the same as the concentration that provokes a response of Y=50.
