What is NS1 protein in dengue?

What is NS1 protein in dengue?

Dengue virus (DENV) nonstructural protein 1 (NS1) is a highly conserved 46-kDa protein that contains 2 glycosylation sites (Asn-130 and Asn-207) and 12 conserved cysteine (Cys) residues. Here, we performed site-directed mutagenesis to generate systematic mutants of viral strain TSV01.

Why is the NS1 protein important?

The NS1 protein acts as a scaffolding protein that anchors the replication complex to the endoplasmic reticulum (ER) membrane and interacts physically with NS4B (4).

Which protein is expressed on surface of dengue infected cell?

Endothelial cell surface expression of protein disulfide isomerase activates β1 and β3 integrins and facilitates dengue virus infection. J Cell Biochem.

What is responsible for dengue virus transmission?

Through Mosquito Bites Dengue viruses are spread to people through the bites of infected Aedes species mosquitoes (Ae. aegypti or Ae. albopictus). These are the same types of mosquitoes that spread Zika and chikungunya viruses.

What is serotype of dengue?

The Dengue Serotypes Dengue infections are caused by four closely related viruses named DEN-1, DEN-2, DEN-3, and DEN-4. These four viruses are called serotypes because each has different interactions with the antibodies in human blood serum.

What are the non-structural proteins of dengue virus?

The 11kb RNA genome of the dengue virus encodes three structural proteins (envelope, pre-membrane, capsid) and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5), all of which are translated as a single polyprotein that is subsequently cleaved by viral and host cellular proteases at specific …

What does NS1 positive mean?

A positive NS1 test result confirms dengue virus infection without providing serotype information. A negative NS1 test result does not rule out infection. People with negative NS1 results should be tested for the presence of dengue IgM antibodies to determine possible recent dengue exposure.

What do you mean by structural protein?

Definition. Therefore, in this focus review, I would like to define a structural protein as “a protein that possesses a characteristic amino acid sequence or motif that repeats and forms a skeleton or contributes to the mechanical properties of a living organism, cell, or material” (Fig. 1).

What are the non structural proteins of dengue virus?

Is dengue transmitted by saliva?

When taking a blood meal, an infected female mosquito injects its saliva into the human host to prevent the host’s blood from clotting and to ease feeding. This injection of saliva infects the host with the dengue virus.

What are the four types of dengue virus?

Dengue is caused by a virus of the Flaviviridae family and there are four distinct, but closely related, serotypes of the virus that cause dengue (DENV-1, DENV-2, DENV-3 and DENV-4). Recovery from infection is believed to provide lifelong immunity against that serotype.

What are 4 types of dengue?

There are four serotypes of dengue viruses, designated as DENV-1, DENV-2, DENV-3, and DENV-4; all are transmitted from human to human through the bite of certain species of Aedes mosquitoes, primarily Ae. aegypti and Ae.