How do you dissolve Red Nile?

How do you dissolve Red Nile?

Nile red is relatively insoluble in aqueous solutions and is first dissolved in acetone, then mixed rapidly with water immediately prior to application to the gel.

Does Nile Blue react with acid or base?

These aromatic nitroso groups are very reactive. Heating of a nitrosylated aminophenol under acidic conditions with hydroxynaphthalene or aminonaphthalene will yield Nile red and Nile blue, respectively, through an acid-catalyzed condensation reaction.

Is Nile red soluble in water?

Nile Red is soluble in methanol (1 mg/mL), ethanol (1mg/mL), water (0.2 mg/mL), and 2-methoxyethanol (1mg/mL).

What is Nile red staining used for?

Nile Red Staining Kit ab228553 is designed for the quantitative, fluorometric detection and measurement of intracellular lipid droplets using fluorescence microscopy, flow cytometry or a fluorescence microplate reader.

Is Nile red soluble in PBS?

Nile red has a solubility of approximately 0.09 mg/ml in a 1:10 solution of DMSO:PBS (pH 7.2) using this method.

How do you store red Nile?

Store at room temperature and protect from light.

Is Nile red and Nile blue the same?

Nile Red has a broad range of emission and can be excited using the 488 nm or 565 nm lasers. Nile Blue is a far-red dye and is excited at 625 nm. Typically, Nile Red fluorescent signal in lipid droplets is observed in the orange-red channels whereas Nile Blue fluorescence is detected in far red channels.

Which compound is used as a quencher for Nile blue fluorescence?

Fluorescence quenching of Nile Blue by amines is thought to be due to electron transfer to the excited dye molecule from the amine electron donor. We used electron transfer quenching of Nile blue byN,N-diethylaniline in propylene glycol as a model system for an interaction which depends exponentially on distance.

What ethnicity is Nile red?

He is of German and Japanese descent. His icons are variations of the Belousov–Zhabotinsky reaction.

Is Nile Red Polar?

Nile red is an uncharged hydrophobic molecule whose fluorescence is strongly influenced by the polarity of its environment. It interacts with many, but not all, native proteins, including beta-lactoglobulin, kappa-casein, and albumin, with a wide range of spectral changes for different proteins.

Does nile red fluorescence?

Nile red exhibits properties of a near-ideal lysochrome. It is strongly fluorescent, but only in the presence of a hydrophobic environment. The dye is very soluble in the lipids it is intended to show, and it does not interact with any tissue constituent except by solution.

What does NileRed do for a living?

First video residing in Montreal, Quebec, Canada. He runs the NileRed YouTube channel and social media accounts.

What is the emission peak at low concentration of Nile red?

At low concentration of Nile red, the hydrophobic core could interact with the dye, leading to an emission peak at 570 nm [34]. Moreover, again at low Nile red concentration (0.5 μM), quenching was found to be quite minor compared with observations at high concentration (4 μM) [84].

What is the role of Nile red in microalgae?

Nile red incorporation into microalgae is a sequential transfer of the dye from the plasma membrane to the lipid droplets. In vitro studies on isolated plasma membranes and isolated lipid droplets from Dunaliella salina compared to in vivo studies highlighted a clear biphasic fluorescence rise [84].

Does Nile red bind to lipid droplets?

Therefore, Nile red does not specifically bind to lipid droplets and these properties may bias the lipid determination with this dye. Additionally, high pigment concentration may also interfere with lipid-induced Nile red fluorescence.

Is there a better alternative to Nile red?

BODIPY 505/515 has been used more recently as a potential alternative to Nile red. In fact, these dyes do not stain all microalgae successfully, even though methods are constantly improving, as reported in the literature.