What is a cis motif?
What is a cis motif?
Cis-regulatory elements are often binding sites for one or more trans-acting factors. To summarize, cis-regulatory elements are present on the same molecule of DNA as the gene they regulate whereas trans-regulatory elements can regulate genes distant from the gene from which they were transcribed.
What are cis-regulatory sequences?
A noncoding DNA sequence in or near a gene required for proper spatiotemporal expression of that gene, often containing binding sites for transcription factors. Often used interchangeably with enhancer.
What does the cis-regulatory module do?
Cis-regulatory modules (CRMs) are DNA sequence elements that have transcriptional regulatory activity. Some CRMs are promoter sequences which bind the basal transcription machinery, consisting of RNA polymerase II and many cofactors, and determine where transcription of each gene is initiated.
What is cis-regulatory analysis?
cis-Regulatory analysis (CRA) is the precise identification of the cis-acting genomic sequences regulating gene transcription. The strengths of this method reflect the use of large clones containing all relevant genomic regulatory sequences to establish a reporter assay with high fidelity.
What are the cis-acting regulatory elements?
cis-Acting regulatory elements are important molecular switches involved in the transcriptional regulation of a dynamic network of gene activities controlling various biological processes, including abiotic stress responses, hormone responses and developmental processes.
What are gene regulatory elements?
A regulatory element is a DNA sequence that certain transcription factors recognize and bind to in order to recruit or repel RNA polymerase. The promoter along with nearby transcription factor binding elements regulate gene transcription.
How do cis-regulatory elements work?
Cis-regulatory elements, such as promoters, enhancers, and silencers, are regions of non-coding DNA, which regulate the transcription of nearby genes. In contrast, trans-regulatory factors regulate (or modify) the expression of distant genes by combining with their target sequences [1, 2].
What is a regulatory module?
In our framework, a regulatory module is a set of genes that are regulated in concert by a shared regulation program that governs their behavior. A regulation program specifies the behavior of the genes in the module as a function of the expression level of a small set of regulators.
What is the name of this cis-acting regulatory element containing the locus?
locus control region
19.1A). A large cis-regulatory element, the locus control region (LCR), is located upstream from the genes, which consists of four regions with enhancer activity (hypersensitive sites 1–4).
What do regulatory genes do?
A regulator gene, regulator, or regulatory gene is a gene involved in controlling the expression of one or more other genes. A regulator gene may encode a protein, or it may work at the level of RNA, as in the case of genes encoding microRNAs.
What is a cis-regulatory element and how does it work?
One cis -regulatory element can regulate several genes, and conversely, one gene can have several cis -regulatory modules. Cis -regulatory modules carry out their function by integrating the active transcription factors and the associated co-factors at a specific time and place in the cell where this information is read and an output is given.
Are novel 6–8 nucleotide motifs associated with shared diurnal regulation?
In this analysis, a Cochran–Mantel–Haenszel based method to control for background variation identified significant enrichment for both expected and novel 6–8 nucleotide motifs in the promoter regions of genes with shared diurnal regulation predicted to function in common physiological activities.
What does the prefix cis mean in biology?
The Latin prefix cis means “on this side”, i.e. on the same molecule of DNA as the gene (s) to be transcribed. CRMs are stretches of DNA, usually 100–1000 DNA base pairs in length, where a number of transcription factors can bind and regulate expression of nearby genes and regulate their transcription rates.
