What does the CMV promoter do?
What does the CMV promoter do?
The CMV promoter is a commonly used promoter for the production of high level recombinant protein in mammalian cells17. However, the expression level of the transgene driven by CMV promoter decreases with extended culture times because of transcriptional silencing, which is associated with DNA methylation18, 19.
Where does the CMV promoter come from?
Due to strong expression and compatibility with numerous cell types, promoters derived from the enhancer region of the cytomegalovirus (CMV) immediate early (IE) promoter are among the most common for general expression vectors.
Is CMV promoter strong?
While there is cell type to cell type variability for all the promoters, CMV promoter is the most variable, being very strong in some cell types (e.g., 293T and CMMT) and rather weak in others (e.g., MRC5 and MSC).
How long is CMV promoter?
Promoters are about 100 to 1000 base pairs long and found upstream of their target genes.
Is EF1a a strong promoter?
The EF‐1α promoter is known as one of the strongest promoters in various mammalian cell lines 32, and the CAG promoter has been used frequently to drive strong gene expression in mammalian cells.
What is AAV Dio?
This AAV expresses DIO-EYFP driven by an ubiquitous EF1a promoter. This arrangement is called DIO (double-floxed inverse ORF), Cre-ON, Flex-rev (reverse), Flex-ON/FlexON, or DIO-AAV/AAV-DIO (double-floxed inverse ORF in AAV).
What activates CMV promoter?
The cytomegalovirus promoter is a very potent promoter commonly used for driving the expression of transgenes, though it gradually becomes silenced in stably transfected cells. Dimethylsulfoxide and 5-Aza-2′-deoxycytidine can activate the cytomegalovirus promoter in both cell types by overlapping mechanisms.
What is T3 primer?
Description. This primer is single-stranded oligonucleotide that has 5′- and 3′-hydroxyl ends. The primer can be used to sequence DNA fragments located downstream from the specific T3 RNA polymerase promoter sequence.
How does the chicken beta actin promoter work in mice?
The chicken β-actin promoter coupled to the cytomegalovirus (CMV) early intermediate minimal enhancer followed by an intron (CAGGS promoter) can yield high-level transgene expression in the mouse [22].
What is CMV early enhancer/chicken beta actin (CAG) promoter?
“The CMV early enhancer/chicken beta actin (CAG) promoter can be used to drive transgene expression during the differentiation of murine embryonic stem cells into vascular progenitors”. BMC Cell Biology. 9: 2. doi: 10.1186/1471-2121-9-2.
What is the beta actin mutation?
The patient carried a heterozygous mutation in the β-actin ACTB gene leading to the expression of a beta-actin mutant at the same levels as the normal β- actin and γ-actin. The mutation resulted in an E346K substitution that did not impair actin polymerization and depolymerization.
What is the pCAGGS promoter?
The resulting synthetic element was used in the pCAGGS expression vector . Although the whole construct is commonly referred to as the “CAG promoter”, it is not a promoter in a strict sense, as it includes a part of the transcribed sequence (the first exon and the first intron of chicken beta-actin gene) and enhancer elements.
