How does PAXgene tube work?

How does PAXgene tube work?

Blood is collected into PAXgene Blood DNA Tubes, which contain a proprietary blend of reagents that both prevents blood coagulation and stabilizes white blood cells. Blood samples can be stored in the tubes for up to 14 days at room temperature.

Who makes PAXgene tubes?

BD PAXgene Blood RNA Tube 2.5mL; Size: 16 x 100mm; Red Hemogard closure:Blood, | Fisher Scientific.

How do you store PAXgene tubes?

To freeze PAXgene™ Blood DNA Tubes, stand them upright in a wire rack. Do not freeze tubes upright in a styrofoam tray as this may cause the tubes to crack. If tubes are to be stored for no longer than 10 weeks, freeze the tubes in the wire rack at -20°C.

Can DNA be extracted from PAXgene RNA tubes?

Our results show that DNA of sufficient quantity and quality for STR analysis can be readily isolated from blood collected in PAXgene RNA tubes. Blood from 25 volunteers was collected into Acid Citrate Dextrose (ACD) tubes or PAXgene Blood RNA tubes (PreAnalytiX GmbH, Switzerland) using an IRB-approved protocol.

What are PAXgene tubes?

The PAXgene® Blood RNA Tube is intended for immediate stabilization of intracellular RNA, thereby yielding accurate and reproducible gene expression data. It is part of the PAXgene Blood RNA System that integrates the key steps of whole blood collection, intracellular RNA stabilization, and RNA purification.

What is EDTA in blood collection tubes?

EDTA stands for Ethylenediaminetetraacetic acid. EDTA functions by binding calcium in the blood and keeping the blood from clotting. BD Vacutainer Plus Blood Collection Tubes contain K2EDTA, which is spray-dried to the walls of the tube.

Do PAXgene tubes need to be refrigerated?

Store the filled PAXgene Blood DNA Tube at room temperature (18–25°C) or refrigerate at 2–8°C. See Performance Characteristics for DNA stability in blood samples at 18–25°C, 2–8°C, 35°C, −20°C, or after freeze-thaw cycles.

What is in PAXgene tube?

The PAXgene Blood RNA Tube contains a proprietary reagent for stabilization of intracellular RNA immediately upon collection. Intracellular RNA is stabilized for up to three days at room temperature or five days at 2-8°C. PAXgene Tubes can be frozen at temperatures of -20°C to -70°C for long-term storage.

Can PAXgene tubes be frozen?

The PAXgene Blood DNA Tubes can be stored at –20ºC for up to 3 months. To freeze PAXgene Blood DNA Tubes, stand them upright in a wire rack or horizontally in a plastic bag. Do not freeze tubes upright in a styrofoam tray as this may cause the tubes to crack.

What is K2 EDTA tubes?

K2 EDTA (dipotassium ethylenediaminetetraacetic acid) is a strong anticoagulant used to store blood for complete blood counts (CBC) in blood collection tubes. Generally, whole blood is stored in these tubes. However, K2 EDTA contains two potassium ions bound to the EDTA molecule.

What is the difference between K2EDTA and k3edta?

The main difference between K2 EDTA and K3 EDTA is that K2 EDTA contains two chelated potassium ions whereas K3 EDTA contains three chelated potassium ions. K2 EDTA and K3 EDTA are two types of anticoagulants used in routine hematological tests.

What are the paxgene blood RNA tubes used for?

The PAXgene Blood RNA Tubes is intended for in vitro diagnostic use. The PAXgene Blood RNA system consists of PAXgene Blood RNA Tubes (available from BD, cat. no. 762165) for blood collection, stabilization, and transport, and the PAXgene Blood RNA Kit for silica-membrane-based RNA isolation and purification in a spin-column format.

How do I freeze paxgene blood DNA tubes?

To freeze PAXgene Blood DNA Tubes, stand them upright in a wire rack or horizontally in a plastic bag. Do not freeze tubes upright in a styrofoam tray as this may cause the tubes to crack.

What is the expected purity and size of DNA extracted from paxgene blood?

Blood samples in PAXgene Blood DNA Tubes can be stored at room temperature (18–25°C) for up to 14 days or at 2–8°C for up to 28 days. For long-term storage, samples should be frozen at –20°C. 3497 – What is the expected purity and size of DNA extracted using the PAXgene Blood DNA kit? The typical A 260/ A 280 ratio is from 1.7 to 1.9.

What is the a260/a280 ratio for paxgene blood DNA?

The PAXgene Blood DNA system consists of PAXgene Blood DNA Tubes, for blood collection and stabilization, and the PAXgene Blood DNA Kit, for DNA purification in a single-tube procedure. DNA isolated using the PAXgene Blood DNA System has an A260 / A280 ratio of 1.7–1.9.