What is Invitrogen TRIzol reagent?
What is Invitrogen TRIzol reagent?
Product information. Invitrogen ™ TRIzol Reagent is a ready-to-use reagent, designed to isolate high quality total RNA (as well as DNA and proteins) from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.
How much does a TRIzol reagent cost?
Each for $348.00. TRIzol Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples.
Why use Invitrogen phasemaker tubes for TRIzol?
Using TRIzol reagents just got simpler, thanks to a new best friend. Invitrogen Phasemaker Tubes create a tight, durable seal between the aqueous and organic phase of your TRIzol mix. Phasemaker Tubes don’t compromise the performance of TRIzol reagents, allowing you to easily pipette off the RNA phase.
How is total RNA isolated by TRIzol® reagent?
Total RNA isolated by TRIzol® Reagent is free of protein and DNA contamination. TRIzol® Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRIzol® Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer
How many biotin binding sites are available for each streptavidin molecule?
The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules. There are two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization. One mg of Dynabeads® M-280 Streptavidin typically binds:
How do you use TRIzol reagent in incubation?
Resuspend the pellet in 1.5–2 mL of 75% ethanol per 1 mL of TRIzol™ Reagent used for lysis. Incubate for 10–20 minutes, mixing occasionally by gentle inversion. Note: The DNA can be stored in 75% ethanol at several months at 4°C. Centrifuge for 5 minutes at 2000 × g at 4°C.
How does the Dynabeads® biotin binder work?
The biotinylated primary antibody may be added to the cell sample (indirect technique) or pre-coated onto the Dynabeads® Biotin Binder (direct technique). Regardless of which of these techniques is used, when the cell sample and the Dynabeads® Biotin Binder are mixed, the beads bind to the target cells.
