What is SQSTM1 gene?

What is SQSTM1 gene?

The SQSTM1 gene provides instructions for making a protein called p62. This protein plays an important role in bone remodeling, a normal process in which old bone is broken down and new bone is created to replace it.

What is selective autophagy?

Selective autophagy contributes to intracellular homeostasis by mediating the degradation of cytoplasmic material such as aggregated proteins, damaged or over-abundant organelles, and invading pathogens. We propose to classify autophagic events into cargo-independent and cargo-induced autophagosome formation events.

How does p62 work?

Under basal conditions, p62 binds the proteasome directly via its PB1 domain. In this mechanism, p62 serves as a shuttling protein for ubiquitinated substrates to the proteasome. Under stress, the binding increases through the involvement of the UBA domain, now associating with the proteasome also via its Ub moieties.

How is ATG7 related to autophagy?

Autophagy related 7 is a protein in humans encoded by ATG7 gene. During the initiation of autophagy, ATG7 acts like an E-1 enzyme for ubiquitin-like proteins (UBL) such as ATG12 and ATG8. ATG7 helps these UBL proteins in targeting their molecule by binding to them and activating their transfer to an E-2 enzyme.

What is non selective autophagy?

Depending on the cargo, autophagy can be selective or nonselective. In nonselective autophagy, bulk portions of the cytoplasm are sequestered by the phagophore for degradation to maintain the nutrient levels in cells. This type of autophagy is frequently observed upon low nutrient levels during starvation.

What is non selective and selective autophagy?

(a) Upon nutrient deprivation, autophagy catabolizes cytoplasmic components nonselectively into autophagosome and mediates recycling and global turnover of cytoplasmic materials.

What is ATG7 gene?

ATG7 (Autophagy Related 7) is a Protein Coding gene. Diseases associated with ATG7 include Spinocerebellar Ataxia, Autosomal Recessive 31 and Danon Disease. Among its related pathways are Autophagy pathway and Cellular Senescence.

Is ATG7 an enzyme?

ATG7 is an autophagy-related E1-like enzyme that is essential for two ubiquitination-like reactions, ATG12-conjugation and LC3-lipidation.

What is the function of the sequestosome 1 gene?

The sequestosome 1 (SQSTM1) gene is located on 5q35.3 and encodes for p62, a ubiquitin binding protein that is important for nuclear factor-κB (NF-κB) signaling, apoptosis, and autophagy. One potential way in which SQSTM1 mutations may lead to disease is through altered autophagy, resulting in pathogenic protein aggregation ( Rea et al., 2014 ).

Is sequestosome-1/p62 upregulated in ULK2 heterozygous mice?

Here we show that Ulk2 heterozygous (Ulk2+/-) mice have upregulated expression of sequestosome-1/p62, an autophagy-associated stress response protein, predominantly in pyramidal neurons of the prefrontal cortex (PFC), and exhibit behavioral deficits associated with the PFC functions, including attenuated sensorimotor gating and impaired cognition

What does the SQSTM1 gene do?

The SQSTM1 gene encodes a 440-amino-acid protein called SQSTM1/p62, which is ubiquitously expressed and contains many structural motifs ( Figure 2 ).

Can sqstm1-gfp (KI/+) mice be used to analyse atherosclerotic plaque burden?

Data show that sequestosome 1 protein p62 deficiency increased atherosclerotic plaque burden in mice with a macrophage-specific ablation of autophagy-related 5 protein ATG5. These results indicate that Sqstm1-GFP (KI/+) mice are a useful tool for analyzing Sqstm1 in living cells and intact animals.