Do you need to perform derivatization technique prior to GC analysis?
Do you need to perform derivatization technique prior to GC analysis?
Derivatization is generally necessary in the analysis by GC of compounds that exhibit low volatility, have poor thermal stability, contain “active” groups that can lead to loss of sample to intermolecular hydrogen bonding or adsorption on the inlet or column, and/or demonstrate poor sensitivity at the detector.
What is a derivatization techniques in GC?
Derivatization Derivatization is the process of chemically modifying a compound to produce a new compound which has properties that are suitable for analysis using a GC or HPLC.
Why is derivatization of sample required in GC?
The main reason for derivatizing is to impart volatility to otherwise nonvolatilecompounds. Derivatization can also be used to decrease volatility to allow analysis of very low molecular weight compounds, to minimize losses in manipulation and to help separate sample peaks from solvent peak.
Are steroids volatile?
Many target analytes, such as steroids, are not intrinsically volatile and will decompose during the transition between the liquid and gas phase inside the injection port or during elution from the column at high temperatures.
What is derivatization reagent?
Derivatization is a technique that usually involves a reaction of the analyte(s) of interest with a particular derivatization reagent. One of the more commonly used in GC analyses involves a reaction that adds a trimethylsilyl (TMS) functional group to the compound. This is also known as trimethylsilylation.
Are steroids soluble in water?
Structure of Steroid Molecules Unlike phospholipids and fats, steroids have a fused ring structure. Although they do not resemble the other lipids, they are grouped with them because they are also hydrophobic and insoluble in water.
What are the advantages of derivatization?
The derivatization often yields a shorter analysis time, improved peak shape and efficiency, enhanced detection sensitivity, and varied enantioselectivities. This is depicted in Figure 4. Another advantage of the derivatization of polar groups is the increased compound volatility and thermal stability.
What is derivatization method?
Derivatization is a technique used in chemistry which converts a chemical compound into a product (the reaction’s derivate) of similar chemical structure, called a derivative. Resulting new chemical properties can be used for quantification or separation of the educt.
