What does fundus autofluorescence show?

What does fundus autofluorescence show?

Fundus autofluorescence (FAF) is a non-invasive imaging technique that detects fluorophores, naturally occurring molecules that absorb and emit light of specified wavelengths [1].

How is fundus autofluorescence done?

Fundus autofluorescence (FAF) is a relatively new, non-invasive imaging modality that has been developed over the past decade. The FAF images are obtained through the use of confocal laser scanning ophthalmoscopy (cSLO).

Can branch retinal vein occlusion be cured?

While BRVO cannot be cured, there are effective treatments that can help patients maintain or improve their visual outcome by reducing the associated macular edema. Treatment options include intravitreal injection (injection of medicine into the eye) and laser.

Can retinal vein occlusion be reversed?

Since retinal vein occlusion cannot be reversed, treatment focuses on protecting your remaining vision. Action and advice to prevent further attacks can also be taken. Treatments include eye massages, glaucoma medication, injections, laser therapy and laser surgery.

What causes autofluorescence?

Autofluorescence (primary fluorescence) is the fluorescence of naturally occurring substances, such as chlorophyll, collagen and fluorite. Most plant and animal tissues show some autofluorescence when excited with ultraviolet light (e.g. light of wavelength around 365 nm).

Is Branch retinal vein occlusion a stroke?

The condition may be preceded by episodes of vision loss known as amaurosis fugax. The cause of CRAO is most commonly a clot or embolus from the neck (carotid) artery or the heart. This clot blocks blood flow to the retina. CRAO is considered a “stroke” of the eye.

Is a retinal vein occlusion a stroke?

Central retinal artery occlusion (CRAO) is a form of acute ischemic stroke that causes severe visual loss and is a harbinger of further cerebrovascular and cardiovascular events.

How do I fix autofluorescence?

Use fluorophores that emit in a wavelength further from the autofluorescence compounds in your sample. Typically, far-red wavelength fluorophores such as CoralLite 647 are best for this. Commercially available reagents such as TrueVIEW (VectorLabs), have been shown to reduce autofluorescence from multiple causes.

How do I get rid of autofluorescence?

Figure 2. Reduction in green and red autofluorescence. Antigen-retrieved human kidney (FFPE tissue) with nuclei stained using DAPI. Green channel/blue channel, without (A) and with (B) TrueVIEW treatment.

What is bacterial autofluorescence?

Autofluorescence is the spontaneous fluorescence of materials without added dyes. Many people are responsible for this work but principal credit goes to Cindy Maddera, who just received her Master’s in my lab. Also contributing were Shelly Theiss, and Amy Huseby.