Are 4T1 cells human?
Are 4T1 cells human?
The 4T1 mammary carcinoma is a transplantable tumor cell line that is highly tumorigenic and invasive and, unlike most tumor models, can spontaneously metastasize from the primary tumor in the mammary gland to multiple distant sites including lymph nodes, blood, liver, lung, brain, and bone The 4T1 tumor has several …
Are 4T1 cells triple negative?
4T1 is a triple-negative mouse breast cancer cell line. Pharmatest utilises a 4T1 cell line with stable expression of green fluorescent protein (GFP) enabling easy and efficient detection of whole-body tumour burden. Use of immunocompetent mice in 4T1 models allows efficacy testing of immonomodulatory compounds.
Is 4T1 metastatic?
When introduced orthotopically, 4T1 is capable of metastasis to several organs affected in breast cancer including lungs, liver and brain, as well as bone [7, 9–11]. 4T1 sibling cell lines with different metastatic properties have been isolated and characterized.
What is MC38?
MC38 (Corbett el al., Can Res, 1975) is a murine colon adenocarcinoma cell line from a C57BL/6 mouse. The cells are adherent and have a fibroblast morphology. MC38 cells will form tumors and metastases post implantation into syngeneic C57BL/6 mice or immunocompromised mice.
What is 4T1 cell line?
4T1 is a breast cancer cell line derived from the mammary gland tissue of a mouse BALB/c strain. 4T1 cells are epithelial and are resistant to 6-thioguanine. In preclinical research, 4T1 cells have been used to study breast cancer metastasis as they can metastasize to the lung, liver, lymph nodes, brain and bone.
What is B16F10?
B16F10 (ATCC® CCL-6475™) is a murine melanoma cell line from a C57BL/6J mouse. It is a subclone of the B16 tumor line, generated by injecting mice with B16 tumor cells, collecting and culturing secondary tumor growths, and injecting them into fresh mice, a total of 10 times.
Can carcinoma be cured?
Most cases of squamous cell carcinoma can be cured when found early and treated properly. Today, many treatment options are available, and most are easily performed at a doctor’s office.
What is MMTV PyMT?
The MMTV-PyMT (mouse mammary tumor virus-polyoma middle tumor-antigen) mouse model of breast cancer is widely used and well characterized. MMTV-PyMT mice develop spontaneous mammary tumors that closely resemble the progression and morphology of human breast cancers [12-14].
What is the morphology of 4T1 cells?
The cells are adherent and have a epithelial morphology. 4T1 cells will form tumors and spontaneous metastases post implantation into syngenic BALB/c mice or immunocompromised mice, which very closely mimic stage IV human breast cancer.
What does 4T1 stand for?
4T1 ( ATCC® CRL-2539 ™) is a murine mammary carcinoma cell line from a BALB/cfC3H mouse. The cells are adherent and have a epithelial morphology. 4T1 cells will form tumors and spontaneous metastases post implantation into syngenic BALB/c mice or immunocompromised mice, which very closely mimic stage IV human breast cancer.
Do 4T1 cells express HER2?
4T1 are typical triple negative breast cancer cell line (ER-, PR-, HER2-), hence they don’t express Her2. They are really mesenchymal and they look completely different from the epithelial HUMAN (!)
Which mice are suitable for in vitro and in vivo experiments with 4T1?
4T1 cells are suitable for in vitro and in vivo experimentation. Both syngenic BALB/c mice and immunocompromised mice can be used for in life studies, and will form tumors and spontaneous metastases following implantation of the cells.
