Can Acinetobacter be oxidase positive?

Can Acinetobacter be oxidase positive?

The genus Acinetobacter, as currently defined, comprises Gram-negative, strictly aerobic, non-fermenting, non-fastidious, non-motile, catalase-positive, oxidase-negative bacteria with a DNA G + C content of 39% to 47%.

Why is Acinetobacter oxidase negative?

Acinetobacter spp. are Gram-negative coccoid rods that are sometimes difficult to destain. They grow aerobically and are oxidase negative using Kovac’s reagent, O-F negative, nonmotile in hanging drop preparations, catalase positive, mostly nitrate negative, and mostly positive for Tween hydrolysis.

Is Acinetobacter a Calcoaceticus?

Acinetobacter calcoaceticus is a bacterial species of the genus Acinetobacter. It is a nonmotile, Gram-negative coccobacillus. It grows under aerobic conditions, is catalase positive and oxidase negative….

Acinetobacter calcoaceticus
Scientific classification
Genus:	Acinetobacter
Species:	A. calcoaceticus
Binomial name

How do you distinguish Acinetobacter species?

Identification. Identification of Acinetobacter species is complicated by lack of standard identification techniques. Initially, identification was based on phenotypic characteristics such as growth temperature, colony morphology, growth medium, carbon sources, gelatin hydrolysis, glucose fermentation, among others.

Is Acinetobacter baumannii urease positive?

Acinetobacter baumannii was confirmed by various phenotypic tests that included: Gram-negative coccobacilli, catalase positive, oxidase negative, non-fastidious, non-fermentative (oxidative), gas negative, H2S negative, hemolysis negative, growth at 44°C, nitrate reduction negative, urease negative, indole negative.

Is Acinetobacter gram-positive or negative?

Acinetobacter baumannii is a pleomorphic aerobic gram-negative bacillus (similar in appearance to Haemophilus influenzae on Gram stain) commonly isolated from the hospital environment and hospitalized patients. A baumannii is a water organism and preferentially colonizes aquatic environments.

Where is Acinetobacter calcoaceticus found?

Species of Acinetobacter are commonly found in soil, water, sewage, food, and milk; they are also part of the normal flora of humans and animals (1). Due to their ubiquitous nature they have become important causes of nosocomial infections, particularly pneumonia and systemic infections in compromised patients.

What is Acinetobacter calcoaceticus baumannii complex?

Acinetobacter baumannii-calcoaceticus complex (ABC) organisms are aerobic gram-negative coccobacillary rods with a natural reservoir in soil and water sources around the world.

How do you confirm Acinetobacter?

For the molecular identification of Acinetobacter species, several methods have been developed, studied and shown to be adequate. This includes DNA–DNA hybridization (DDH), DNA sequence-based analysis and restriction analysis of PCR amplified DNA sequences (PCR-restriction fragment length polymorphism [PCR-RFLP]).

Is a baumannii oxidase positive?

baumannii is one of the most challenging bacterial pathogens because of its unique antibiotic resistance characteristics. The genus Acinetobacter includes non-lactose-fermenting, catalase-positive, non-motile, non-fastidious, oxidase-negative, and aerobic Gram-negative coccobacilli.

Is Acinetobacter gram positive or negative?

Is Acinetobacter calcoaceticus oxidase positive?

Acinetobacter calcoaceticus. It grows under aerobic conditions, is catalase positive and oxidase negative. It is part of the normal human intestinal flora. Together with A. baumannii, it is referred to as the A. calcoaceticus-A. baumannii complex, which is relatively simple to identify based on the beforementioned phenotypic characteristics.

Is genus Acinetobacter Gram positive or negative?

Genus Acinetobacter is a group of gram-negative, non-fermentative, aerobic and non-motile rods. According to the older nomenclature, Acinetobacter was represented by a single species, Acinetobacter calcoaceticus, included in the Neisseriaceae family.

When was Acinetobacter first identified?

The first ‘Acinetobacter’ microorganisms were identified in 1911 by the Dutch microbiologist, Beijerinck, who assigned the bacteria the name Micrococcus calcoaceticus following their isolation from soil (Beijerinck, 1911; Baumann et al., 1968).

Is Acinetobacter baumannii motile or aerobic?

Acinetobacter baumanniiis a Gram-negative bacillus that is aerobic, pleomorphic and non-motile.