Does Klebsiella pneumoniae have a capsule?
Does Klebsiella pneumoniae have a capsule?
The capsular polysaccharide and type 1 fimbriae are two of the major surface-located virulence properties associated with the pathogenesis of Klebsiella pneumoniae. The capsule is an elaborate polysaccharide matrix that encases the entire cell surface and provides resistance against many host defense mechanisms.
What is the shape and arrangement of Klebsiella pneumoniae?
Klebsiella pneumoniae is a rod-shaped, Gram-negative bacterium that belongs to the family Enterobacteriaceae and is widely distributed in the mouth, skin, intestines, hospital settings, and medical devices.
Is Klebsiella pneumoniae capsule positive or negative?
Klebsiella pneumoniae is a gram-negative, encapsulated, non-motile bacterium found in the environment and has been associated with pneumonia in patient populations with alcohol use disorder or diabetes mellitus. The bacterium typically colonizes human mucosal surfaces of the oropharynx and gastrointestinal (GI) tract.
What is the shape of Klebsiella pneumoniae?
Klebsiella is a Gram-negative, non-motile, and rod-shaped bacteria.
What is capsule stain?
Capsule stain is a type of differential stain which uses acidic and basic dyes to stain background & bacterial cells respectively so that presence of capsule is easily visualized. Capsule is synthesized in the cytoplasm and secreted to the outside of the cell where it surrounds the bacterium.
Is S pneumoniae capsule forming?
Streptococcus pneumoniae (the pneumococcus) is an important human pathogen. Its virulence is largely due to its polysaccharide capsule, which shields it from the host immune system, and because of this, the capsule has been extensively studied.
What color is Klebsiella pneumoniae?
Laboratory Identification of Klebsiella pneumoniae colonies are pink (LF), mucoid (usually), and 3 to 4 mm in diameter. Colonies on Hektoen enteric agar and XLD are yellow. Large, mucoid, glistening pink colonies on a MacConkey agar plate, typical of the colonies produced by many Klebsiella and Enterobacter spp.
What are the characteristics of Klebsiella?
The species of Klebsiella are all gram-negative and usually non-motile. They tend to be shorter and thicker when compared to others in the family Enterobacteriaceae. The cells are rods in shape and generally measures 0.3 to 1.5 µm wide by 0.5 to 5.0 µm long.
Why is the capsule considered a virulence factor?
Capsules contain water which protects the bacteria against desiccation. They also exclude bacterial viruses and most hydrophobic toxic materials such as detergents and help the cells to adhere to surfaces. The capsule is considered a virulence factor because it enhances the ability of bacteria to cause disease.
Is Klebsiella pneumoniae bacilli or cocci?
Klebsiella pneumoniae is a Gram-negative, non-motile, encapsulated, lactose-fermenting, facultative anaerobic, rod-shaped bacterium….
| Klebsiella pneumoniae | |
|---|---|
| Order: | Enterobacterales |
| Family: | Enterobacteriaceae |
| Genus: | Klebsiella |
| Species: | K. pneumoniae |
What are the two different methods of capsule staining?
This unit describes two methods of capsule staining. The first is a wet-mount method using india ink; the capsule is visualized as a refractile zone surrounding a cell. The second is a direct-staining dry-mount method that precipitates copper sulfate and leaves the capsule as a pale blue zone.
What are some types of molecules that may be found in a capsule?
What biological molecules can make up the bacteria capsule or glycocalyx? Capsules are composed of polysaccharides or proteins.
How many types of capsular types are there in Klebsiella pneumoniae?
Capsule is an important virulence factor in bacteria. A total of 78 capsular types have been identified in Klebsiella pneumoniae. However, there are limitations in current typing methods. We report here the development of a new genotyping method based on amplification of the variable regions of the …
What is CPS Klebsiella pneumoniae?
Capsule polysaccharide (CPS) Klebsiella pneumoniae produces an acidic capsule polysaccharide that is important for survival in the host. K. pneumoniae has been classified historically by capsule (K antigen) serotyping and to date 79 capsule types have been identified.
What is the virulence of Klebsiella pneumoniae?
As discussed previously, K. pneumoniae is most commonly an opportunistic pathogen and consistent with that lifestyle, produces relatively few virulence factors per se [6]. Nevertheless, some strains of K. pneumoniae are adept at evading recognition by the host innate immune system and are resistant to phagocytosis.
Can WZC genotyping be used for capsular typing of Klebsiella pneumoniae?
Thus, the wzc genotyping system is a simple and useful method for capsular typing of K. pneumoniae. Citation: Pan Y-J, Lin T-L, Chen Y-H, Hsu C-R, Hsieh P-F, Wu M-C, et al. (2013) Capsular Types of Klebsiella pneumoniae Revisited by wzc Sequencing.
