How do you dissolve deoxycholic acid?

How do you dissolve deoxycholic acid?

Deoxycholic acid (DCA) is supplied as a crystalline solid. A stock solution may be made by dissolving the DCA in the solvent of choice, which should be purged with an inert gas. DCA is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF).

How do you remove sodium deoxycholate?

Sodium deoxycholate was removed after tryptic digestion by acidification using 0.5% formic acid and centrifugation at 14 000 g (RA-300, Kubota 7820) for 15 min at ambient temperature.

What is the function of sodium deoxycholate?

Sodium deoxycholate, the sodium salt of deoxycholic acid, is often used as a biological detergent to lyse cells and solubilise cellular and membrane components.

What is deoxycholic acid made from?

Deoxycholic acid is a secondary bile acid produced by intestinal bacteria acting on primary bile acids secreted by the liver. Deoxycholic acid is also known as deoxycholate and cholanoic acid. Deoxycholic acid emulsifies fats to aid their intestinal absorption.

How do you dissolve sodium cholate?

Sodium deoxycholate precipitates in low pH. Ideally you should dissolve it in a solution buffered at slightly basic pH (~7.4-8). If this condition is not suitable for you always prepare a fresh solution in water and check/adjust the pH accordingly.

Does cholic acid dissolve in water?

Cholic acid, also known as 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid is a primary bile acid that is insoluble in water (soluble in alcohol and acetic acid), it is a white crystalline substance.

Is sodium deoxycholate a detergent?

Thermo Scientific Sodium Deoxycholate is an ionic detergent that is especially useful for disrupting and dissociating protein interactions. Sodium deoxycholate (deoxycholic acid) is a water-soluble, bile-acid, ionic detergent commonly used in protein methods.

Is sodium deoxycholate toxic?

Ingestion Harmful if swallowed. Harmful if absorbed through skin. May cause skin irritation. Eyes May cause eye irritation.

What is the purpose of deoxycholic acid?

Deoxycholic acid is a manmade form of a substance your body makes that helps to absorb fats. Deoxycholic acid works by destroying fat cells where it is injected into the body. Deoxycholic acid is used to help decrease the appearance of fat that hangs below the chin, sometimes called a double-chin.

Is deoxycholic acid carcinogenic?

DOC is a natural endogenous agent increased in the colonic contents of humans in response to a high fat diet. Our results show that DOC can act as a carcinogen.

Is sodium deoxycholate the same as deoxycholic acid?

Sodium deoxycholate (deoxycholic acid) is a water-soluble, bile-acid, ionic detergent commonly used in protein methods.