How many types of HPLC columns are there?
How many types of HPLC columns are there?
High performance liquid chromatography (HPLC) is a type of liquid chromatography that uses a liquid moblie phase. The same basic principals from gas chromatography are applied to liquid chromatography. There are three basic types of liquid chromatographic columns: liquid-liquid, liquid-solid, and ion-exchange.
Which brand HPLC is best?
Re: HPLC brand Waters and Agilent are good, or maybe the best two brands of HPLC. But in my country, the third is Shimadzu HPLC, a japanese brand, with good quality and much lower price then waters and agilent.
What is Rp C18 column?
C18 columns are HPLC (high performance liquid chromatography) columns that use a C18 substance as the stationary phase. C18 simply means that the molecules contain 18 carbon atoms, so the other atoms in the molecule can vary, leading to significantly different substances.
What is the cost of HPLC?
You can get a “bare bones” HPLC for about $20K, but $40-50K is more typical by the time you throw in automation features and data analysis software. Conventional electrophoresis equipment is much cheaper, but a modern capillary electrophoresis setup isn’t.
What is Shimadzu HPLC?
Shimadzu provides a range of liquid chromatography systems from HPLC (High Performance Liquid Chromatography) to UHPLC, preparative systems, and more. We also offer solutions for supercritical fluid chromatography and ion chromatography.
What is the difference between C8 and C18 column?
C18 has 18 carbon atoms while C8 has only 8 carbon atoms. C18 has a longer carbon chain, but C8 has a shorter one. C18 has higher retention while C8 has shorter retention. C18 has higher hydrophobicity, but C8 has a lower hydrophobicity.
What is pore size in HPLC column?
Pore size is the average size of a pore in a porous packing. Its value is typically expressed in angstroms. Therefore, the pore size of the packing material in your HPLC column is important, since the molecules must ‘fit’ into the porous structure in order to interact with the stationary phase.
What are the different column types available in HPLC?
The following column types are available on the market: 1. Silica gel HPLC column: Silica gel is the earliest chromatographic column packing used in liquid chromatography. It has a wide range of applications. However, it can only be used under the condition of pH 2.0-7.5, and the use temperature must be less than 60 degrees.
Why choose Hawach as your HPLC column manufacturer?
As an HPLC columns manufacturer, Hawach has a dynamic, high-quality sales team and a comprehensive technical and experienced after-sales service team; we will provide you with more application support.
Where can I buy HPLC analytical columns in Thane?
More A-71, Road No 22, Wagle Industrial Estate, Opp.Lodha Grandezza, Near Spraytech Circle, (Tata Motors Bus stop), Thane, Maharashtra, India Our firm is offering HPLC Analytical Columns to our consumers. The column temperature should be high enough so that the analysis i…
What is a reversed phase LC column?
Thermo Scientific reversed phase LC columns are available in an array of chemistries to optimize separations and provide enhanced retention or changes in elution order. Reversed phase columns are one of the most popular modes in HPLC analysis. Want a C18 column that’s right for your application?
