How to dilute BODIPY?

How to dilute BODIPY?

Dissolve 1.3 mg BODIPY in 1 ml DMSO and can be stored at −20 °C. 2 μM BODIPY staining solution can be prepared by diluting stock solution 1:2,500 in PBS.

Can bodipy be fixed?

We routinely add BODIPY 493/503 from a 1 mg/ml stock to fixed or living cells at a final concentration of 1 μg–10 μg/ml; followed by at least a 1-h incubation at 37°C. The final concentration of BODIPY 493/503 depends on fat droplet size, the larger the fat droplets, the less stain is needed.

What is Nile red staining?

Nile red (also known as Nile blue oxazone) is a lipophilic stain. Nile red stains intracellular lipid droplets yellow. Nile red has also been used as part of a sensitive detection process for microplastics in bottled water.

What is LipidTOX?

HCS LipidTOX™ Deep Red neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader.

How do you store bodipy?

For Invitrogen BODIPY 493/503 dye (Cat. No. D3922), one may make a stock solution of 1 mg/mL (3.8 mM) in either absolute ethanol or anhydrous DMSO. After resolubilizing in either solvent, store the stock solution frozen, desiccated, and protected from light.

Is bodipy hydrophobic?

BODIPY FL dye is a green fluorescent dye with unique hydrophobic properties ideal for staining lipids, membranes, and other lipophilic compounds.

What is bodipy used for?

BODIPY dyes have been used extensively in recognition and imaging applications owing for their improved stability toward photobleaching as well as high quantum yield (Φ) and high molar absorption coefficient (ɛ) values.

Does bodipy stain cholesterol?

The BODIPY-cholesterol assay can detect and quantify cholesterol efflux in capacitating conditions.

What ethnicity is Nile red?

He is of German and Japanese descent. His icons are variations of the Belousov–Zhabotinsky reaction.

What does bodipy stand for?

boron-dipyrromethene
2. ; specifically, the compound 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene in the IUPAC nomenclature. The common name is an abbreviation for “boron-dipyrromethene”. It is a red crystalline solid, stable at ambient temperature, soluble in methanol.

Is BODIPY a fluorescent?

Invitrogen BODIPY FL dye is a bright, green-fluorescent dye with similar excitation and emission to fluorescein (FITC) or Invitrogen Alexa Fluor 488 dye. It has a high extinction coefficient and fluorescence quantum yield and is relatively insensitive to solvent polarity and pH change.

Is BODIPY cell permeable?

These reports indicate that BODIPY dyes without any hydro- philic functionality exhibit membrane permeability, but cannot avoid localization into the intracellular membrane.

What is BODIPY® 493/503?

The resulting BODIPY® 493/503 conjugates exhibit bright fluorescence, narrow emission bandwidths, and relatively long excited-state lifetimes, which can be useful for fluorescence polarization assays and two-photon excitation (TPE) microscopy. This reactive dye contains a C 3 alkyl spacer between the fluorophore and the NHS ester group.

Is the BODIPY (493/503) dye an acceptable alternative for storing LDS in skeletal muscle?

We believe that the use of the BODIPY (493/503) dye is an acceptable alternative and, under certain conditions, a simpler method for visualizing LDs stored within skeletal muscle. 1. Introduction Triacylglycerol (aka triglyceride, or TAG) storage is typically at its highest appreciable amounts in white adipose tissue.

How can I make a stock solution of Invitrogen BODIPY 493/503 dye?

What can be used to make a stock solution of Invitrogen BODIPY 493/503 dye (Cat. No. D3922)? For Invitrogen BODIPY 493/503 dye (Cat. No. D3922), one may make a stock solution of 1 mg/mL (3.8 mM) in either absolute ethanol or anhydrous DMSO.

Can BODIPY be used as an alternative to Oro for LDS?

An advantage of the dye is that it requires little effort to place into solution and, unlike ORO, does not need to be made “fresh” for each use. The purpose of this paper is to demonstrate the use of BODIPY (493/503) to image LDs within skeletal muscle fibers as an alternative to ORO.