Is DsRed monomeric?
Is DsRed monomeric?
DsRed-Monomer is a monomeric protein. Panel A. Panel B.
What is red fluorescent dye?
Texas Red or sulforhodamine 101 acid chloride is a red fluorescent dye, used in histology for staining cell specimens, for sorting cells with fluorescent-activated cell sorting machines, in fluorescence microscopy applications, and in immunohistochemistry. This is one of the advantages of conjugating with Texas Red vs.
What is Tdtom?
tdTomato is an exceptionally bright red fluorescent protein—6X brighter than EGFP. tdTomato’s emission wavelength (581 nm) and brightness make it ideal for live animal imaging studies. The tdTomato fluorescent protein is as photostable as mCherry (Shaner et al. 2004).
What makes RFP red?
Red fluorescent protein (RFP) is a fluorophore that fluoresces red-orange when excited. Several variants have been developed using directed mutagenesis. The original was isolated from Discosoma, and named DsRed. Others are now available that fluoresce orange, red, and far-red.
Are DsRed and RFP the same?
What does DsRed stain?
Our data demonstrated that DsRed-NLS can be used to stain the nuclei of live cells in a manner that is compatible with the use of yEGFP3 and confocal laser-scanning microscopy. This has proven to be difficult with the existing chemical fluorescent dyes for the nucleus (M.
What is the DsRed-monomer sequence?
The DsRed-Monomer sequence contains 45 amino acid substitutions but maintains a spectral profile virtually identical to other DsRed fluorescent proteins. The DsRed-Monomer fluorescent protein is extremely stable, allowing you to monitor fluorescence over extended periods of time.
Is the DsRed-monomer patented in the US?
The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,005,511 and 7,393,923. pDsRed-Monomer-Actin encodes DsRed-Monomer, a monomeric mutant of the Discosoma sp. red fluorescent protein DsRed.
What is the plvx-DsRed-monomer-c1 vector?
The pLVX-DsRed-Monomer-C1 vector is an HIV-1-based lentiviral expression vector that can produce lentivirus that efficiently transduces both dividing and non-dividing mammalian cells. This vector expresses a monomeric mutant of the Discosoma sp. red fluorescent protein (DsRed).
What is the DsRed-monomer fluorescent protein?
The DsRed-Monomer sequence contains 45 amino acid substitutions but maintains a spectral profile virtually identical to other DsRed fluorescent proteins. The DsRed-Monomer fluorescent protein is extremely stable, allowing you to monitor fluorescence over extended periods of time. However, it is not as bright as mCherry or tdTomato.
