Is resazurin toxic to cells?

Is resazurin toxic to cells?

Resazurin, introduced as a cell viability indicator under the trade name alamarBlue(®), is generally regarded as nontoxic when used according to manufacturer’s suggested shorter-term incubation time specifications. However, problems arise when exposure times are extended to longer-term cultures on the order of days.

What is the function of resazurin?

Resazurin is used to measure the amount of aerobic respiration in streams. Since most aerobic respiration occurs in the stream bed, the conversion of resazurin to resorufin is also a measure of the amount of exchange between the water column and the stream bed.

What enzymes reduce resazurin?

Resazurin (blue) diffuses into cells where it is irreversibly reduced by diaphorase enzymes to a highly fluorescent (pink) compound, resorufin.

How can I reduce my blue Alamar?

alamarBlue can be reduced by FMNH2, FADH2, NADH, NADPH, and cytochromes, since their midpoint potentials are lower than that for alamarBlue.

What is a resazurin test for milk?

The Resazurin test is designed for assessing the quality of raw bulked milk. Resazurin gives milk a characteristic blue colour and the test is based on the ability of bacteria in the milk to reduce the blue dye.

Why is resazurin added to milk?

What is a resazurin assay?

The resazurin assay is commonly used to quantify the number of live cells in a sample, and to monitor cell viability / cytotoxicity. It is a fast, simple, accurate and homogeneous / no-wash high throughput assay that can be used to monitor cells for up to 24-48 hours.

Is resazurin light sensitive?

The resazurin dye (blue) in the CellTiter-Blue® Reagent and the resorufin product (pink) are light-sensitive. Prolonged exposure of the CellTiter-Blue® Reagent to light will result in increased background fluorescence in the assay and decreased sensitivity.

Is Alamar blue light sensitive?

alamarBlue® should be stored in the dark, since the compound is light sensitive (Table 2). The product may be stored for 12 months at room temperature.

Is Alamar blue fluorescence?

Resazurin, the active ingredient of alamarBlue reagent, is a non-toxic, cell-permeable compound that is blue in color and virtually non-fluorescent. Upon entering living cells, resazurin is reduced to resorufin, a compound that is red in color and highly fluorescent.

Why is Resazurin added to milk?