What activates CDT1?
What activates CDT1?
The human CDT1 gene has three putative E2F responsive elements in its promoter region, is activated by E2F with peak expression in late G1, and is inhibited by the Rb tumor suppressor [117].
What are CDC6 and CDT1?
CDC6 is an ATP binding protein and a member of the pre-replicative complex (pre-RC) together with the origin recognition complex (ORC), Cdt1 and the MCM complex (containing MCM2-7p). CDC6 assembles after ORC in an ATP dependent manner and is required for loading MCM proteins onto the DNA.
What does CDT1 do?
The CDT1 gene provides instructions for making a protein that is important in the copying of a cell’s DNA before the cell divides (a process known as DNA replication). The protein produced from this gene is one of a group of proteins known as the pre-replication complex.
How is CDT1 regulated?
Regulation : CDT1 is regulated either by cell cycle dependent proteolysis during S and G2 phase or by geminin. CDT1 activity is also inhibited by the tight binding of geminin that blocks the ability of CDT1 to load MCM2-7 onto DNA.
What are the two 2 the protein known as CDT1?
CDT1 (Chromatin Licensing And DNA Replication Factor 1) is a Protein Coding gene. Diseases associated with CDT1 include Meier-Gorlin Syndrome 4 and Meier-Gorlin Syndrome 1. Among its related pathways are Cell Cycle, Mitotic and Mitotic G1-G1/S phases.
Why is Cdc6 important in helicase loading?
ATP hydrolysis by Cdc6 and ORC plays distinct and ordered roles in helicase loading. Cdc6 is activated to bind and hydrolyze ATP when it associates with origin-bound ORC, and this hydrolysis event is required to observe Mcm2–7 loading (Perkins and Diffley 1998; Randell et al.
What is DDK protein?
The yeast Dbf4-dependent kinase (DDK) (composed of Dbf4 and Cdc7 subunits) is an essential, conserved Ser/Thr protein kinase that regulates multiple processes in the cell, including DNA replication, recombination and induced mutagenesis.
What is the function of B clamp and DNA polymerase?
A DNA clamp, also known as a sliding clamp or β-clamp, is a protein complex that serves as a processivity-promoting factor in DNA replication. As a critical component of the DNA polymerase III holoenzyme, the clamp protein binds DNA polymerase and prevents this enzyme from dissociating from the template DNA strand.
