What affects the heart rate of Daphnia?
What affects the heart rate of Daphnia?
The addition of caffeine increased the heart beats per minute of the Daphnia in each trial. When the caffeine was added, their speed and movement remained the same. A paired T-test was conducted to compare the effect of 5%ethanol on the heart rate of Daphnia.
How does caffeine affect heart rate experiment?
Contrary to the hypothesis, caffeine did not increase the heart rate; however, analysis of the data showed a slight decrease in heart rate over increasing caffeine concentration.
How does sugar affect Daphnia heart rate?
The results of the experiment showed that high concentrations of sucrose, salt, and caffeine drastically increased the Daphnia’s heart rate. As the concentrations were diluted, the heart rate of the Daphnia decreased, reaching closer to the original heart rate.
How does caffeine affect heart rate biology?
In humans, caffeine acts as a stimulant drug, causing increased amounts of stimulatory neurotransmitters to be released. At high levels of consumption caffeine has been linked to restlessness, insomnia and anxiety, causing raised stress and blood pressure. This can lead to heart and circulation problems.
How does caffeine affect Daphnia magna?
Both caffeine and dopamine were found to instantly increase the heart rate of Daphnia in a dose-dependent manner. Interestingly, caffeine synergized with dopamine to increase Daphnia’s heart rate.
How does caffeine change the cardiac output of the Daphnia?
An increase in caffeine concentration results in a faster heart rate (more beats per minute, bpm). Therefore, heart rate increases.
How does caffeine affect Daphnia heart rate?
How does coffee affect Daphnia heart rate?
Caffeine showed a similar effect on Daphnia’s heart rate at a 10-times lower concentration than dopamine (28.5% increase at 0.08 mg/ml, Figure 2). Dopamine is the precursor of norepinephrine, and has been shown to augment heart activity by affecting beta-adrenergic receptors, in the case of a canine model 16.
How does salt affect Daphnia heart rate?
As salt levels increase, the amount of dissolved oxygen decreases. This means that Daphnia need to breathe more to get enough oxygen, and this means an increase in heart rate (Tan, 2015).
Does caffeine affect heart rate of Daphnia?
Do Daphnia have a heart?
Daphnia have a myogenic heart, exhibiting responses comparable to that of the human heart to a range of established therapeutics, and displaying varying arrhythmias on exposure to pro-arrhythmic agents.
Does caffeine increase heart rate in Daphnia?
Caffeine at 0.1%%, 0.5%%, and 2%% (w//v) had no convincing effect on heart rate. Of the three agents tested, nicotine’s peculiar effects make it the least useful in an educational setting. Caffeine could be used to emphasize the need for blind observers because it does not increase heart rate in Daphnia.
How do you test for caffeine in Daphnia?
Count heart rate of the Daphnia when caffeine concentration is 0M (in distilled water). Remove 1 Daphnia with a pipette and place it in a cavity slide under a microscope.
Do chemical agents affect heart rate in crustacean Daphnia magna?
Students commonly test the effects of chemical agents on the heart rate of the crustacean Daphnia magna, but the procedure has never been optimized. We determined the effects of three concentrations of ethanol, nicotine, and caffeine and of a control solution on heart rate in Daphnia.
How do you measure the heart rate of a Daphnia?
If the Daphnia isn’t too large, you may want to use a cover slip to hold it in place. Add two or three drops of your 1% solution. Using your stopwatch, count how many times the Daphnia’s heart beats in one minute. Return the Daphnia to the jar. Do this at least 10 more times, and record heart rates. Find the average heart rate with a 1% solution.
