What are microtiter plates used for?
What are microtiter plates used for?
Microtiter plates are most frequently used for screenings and cultivation of large sample amounts. In order to perform an immunoassay (e. g. ELISA = Enzyme Linked Immuno Sorbent Assay) the bottoms of microtiter plate wells are coated with antibodies to detect specific substances within samples.
What kind of Plates to use for ELISA?
ELISAs are typically performed in 96-well or 384-well polystyrene plates, which passively bind antibodies and proteins.
How do you coat an ELISA plate?
Coating of Capture Antibody
- Appropriately dilute the capture or coating antibody in carbonate-bicarbonate buffer or PBS.
- Pipette 0.2 ml of the diluted capture antibody to each well of a microtiter plate.
- Incubate the plate (covered) for 1 hour at 37 °C.
- Remove the coating solution.
What are MaxiSorp plates?
Immuno plates, MaxiSorp surface—These plates are designed for solid phase immuno-assays and have a polystyrene surface with high affinity for polar groups and hydrophilic molecules.
What are microplates made of?
Microplates are manufactured in a variety of materials. The most common is polystyrene, used for most optical detection microplates. It can be coloured white by the addition of titanium dioxide for optical absorbance or luminescence detection or black by the addition of carbon for fluorescent biological assays.
Can TC treated plates be used for ELISA?
Tissue Culture-treated Polystyrene Non-specific background tends to be difficult to control. We advise that surfaces specified as Tissue Culture-treated not be used for ELISA or similar procedures.
Why must you incubate the ELISA plate at 37 degrees Celsius?
All Answers (5) In general ELISA requires to incubate at 37 degree up to 1 to 2 hrs for secondary HRP antibody to bind to antigen.
How long does it take to coat ELISA?
It is dependent upon time, temperature, and the pH of the coating buffer, as well as the concentration of the coating agent. Typical coating conditions involve adding 50-100 µl of coating buffer, containing antigen or antibody at a concentration of 1-10 µg/ml, and incubating overnight at 4 C or for 1-3 hours at 37 C.
How do you optimize ELISA?
How to optimize your ELISA experiment
- Capture Antibody Concentration. Prepare different concentrations of the capture antibody in coating buffer.
- The Blocking Buffer.
- The Standard Diluent.
- Sample Concentration.
- The Detection Antibody Concentration.
- The Enzyme Conjugate Concentration.
- Signal Detection.
What are Nunc plates?
Thermo Scientific™ Nunc MicroWell 96-Well Microplates are optimized for robotics, automated readers and liquid handling systems. 96-well microplates are used for antibiotic screens, cell-based assays and screening compounds. The flat bottom shape is ideal for microscopic and optical measurements.
What is the surface area of a 96 well plate?
96-Well Plates
| Cat.No. | Brand | Growth Area |
|---|---|---|
| 83-3300 | Corning® | 0.32cm2 |
| 83-3596 | Corning® | 0.32cm2 |
| 83-3997 | Corning® | 0.32cm2 |
| 83-3598 | Corning® | 0.32cm2 |
What are the different types of microplate materials used in Elisa?
Streptavidin, antibody, nickel, and other ligand coated microplates for affinity binding of proteins and other biomolecules for ELISA and other assay techniques: Antibody-Binding Plates, including Protein A/G/L-coated plates and secondary antibody (GAM, GAR)-coated plates
What are Nunc-immuno 96 microwell solid plates used for?
Nunc-Immuno 96 MicroWell solid plates are intended for quantitative and qualitative solid phase immuno assays (i.e. ELISA) and binding assays. Nunc-Immuno™ 96 MicroWell™ solid plates have been used for quantitative and qualitative solid phase immuno assays, such as ELISA.
What plasticware do I need to complete my Elisa protocols?
We also offer all the plasticware needed to complete your ELISA protocols. Treated “blank” microplates for passive adsorption (coating) of antibodies, proteins, and other biomolecules for ELISA and other assay techniques: Hydrophilic, Protein-Binding Plates, for most antibodies, glycans, glycoproteins, and soluble proteins
What is the maxisorp™ high protein-binding capacity 96 well ELISA plate?
The Nunc MaxiSorp™ high protein-binding capacity 96 well ELISA plates are recommended for use in capturing immunoglobulins found in eBioscience Ready-SET-Go! ELISA kits, as well as other matched antibody pairs for ELISA. Maxisorp plates have a protein-binding capacity of approximately 600-650 ng IgG/cm2.
