What do type 2 topoisomerase do?
What do type 2 topoisomerase do?
Topoisomerases are ubiquitous enzymes, found in all living organisms. In humans, topoisomerase II is a chemotherapy target. In prokaryotes, gyrase is an antibacterial target. Indeed, these enzymes are of interest for a wide range of effects.
Is topoisomerase a DNA binding protein?
A DNA-topoisomerase-II-binding protein with eight repeating regions similar to DNA-repair enzymes and to a cell-cycle regulator.
What is the function of the protein topoisomerase?
DNA topoisomerases solve the topological problems associated with DNA replication, transcription, recombination, and chromatin remodeling by introducing temporary single- or double-strand breaks in the DNA.
What is the difference between topoisomerase I and II?
Topoisomerase I and II are methods of dealing with supercoiled DNA. Topoisomerase I cuts one strand in the double-stranded DNA and no ATP is required for its function. On the other hand Topoisomerase, II cuts both strands in DNA and needs ATP for its activity. This is the key difference between Topoisomerase I and II.
What enables the DNA topoisomerase to nick DNA?
In general, DNA is wound around a basic protein called histone in a tightly coiled or supercoiled formation (Durand-Dubief et al. Type II DNA topoisomerases (or DNA topoisomerase II) temporarily break the DNA strand, allowing both ends to rotate within the enzyme freely. This mechanism allows the DNA to unwind.
What Bond does topoisomerase break?
Topoisomerase breaks a covalent bond in the backbone of one parental strand. ( Topoisomerase relieves the strain caused by unwinding of the DNA by helicase.
What happens if DNA topoisomerase is inhibited?
Topoisomerase inhibitors influence these essential cellular processes. These topoisomerase-DNA-inhibitor complexes are cytotoxic agents, as the un-repaired single and double stranded DNA breaks that they cause can lead to apoptosis and cell death.
What is the function of DNA Topoisomerase 2 beta?
A DNA topoisomerase 2-beta that is encoded in the genome of human. Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand.
What does TOPBP1 do for E2F1?
“TopBP1 recruits Brg1/Brm to repress E2F1-induced apoptosis, a novel pRb-independent and E2F1-specific control for cell survival.” Cited for: FUNCTION, INTERACTION WITH E2F1; SMARCA2; SMARCA4 AND THE SWI/SNF CHROMATIN REMODELING COMPLEX. “TopBP1 activates the ATR-ATRIP complex.” Cited for: FUNCTION.
How does TOPBP1 work with BRG1?
“TopBP1 recruits Brg1/Brm to repress E2F1-induced apoptosis, a novel pRb-independent and E2F1-specific control for cell survival.” Cited for: FUNCTION, INTERACTION WITH E2F1; SMARCA2; SMARCA4 AND THE SWI/SNF CHROMATIN REMODELING COMPLEX.
