What does acid phosphatase do in wheat germ?
What does acid phosphatase do in wheat germ?
Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction pr ocesses of the cell.
Is acid phosphatase a competitive inhibitor?
Potent competitive inhibition of plant acid phosphatases by phosphate analogues such as arsenate, molybdate, tar- tratc and vanadate has also been reported.” ” Feedback inhibition of acid phosphatases by phosphate may represent a general form of cellular regula- tion of these enzymes.
How does phosphate inhibit acid phosphatase?
Phosphate and arsenate are well known as inhibitors of acid phosphatase activity; the inhibition of PNPPase activity by phosphate and the more efficient inhibition by sodium arsenate, which is its structural analog, suggest a common mode of binding to the active site [40].
What type of inhibitor is phosphate to alkaline phosphatase?
Enzymes and Enzyme Regulation In the alkaline phosphatase reaction, in which hydrolysis of a wide variety of organic monophosphate esters into the corresponding alcohols (or phenols) and inorganic phosphates occurs, the inorganic phosphate acts as a competitive inhibitor.
What is the optimum pH for wheat germ acid phosphatase some research needed here )? What was your information source?
The molecular mass of wheat germ APase is 58 kDa (gel filtration), the pH Optimum is 5.7, the pH Range is 4.0–7.0 and the optimal temperature is 45 °C.
What is the optimum pH for acid phosphatase?
The pH optimum for acid phosphatase activity was 5.8 for 67% of the isolates and 4.8 for 33%. All of the isolates showed peak phosphatase activity at 37 degrees C.
What type of inhibitor is NAF?
By Lineweaver-Burk plot, sodium fluoride shows non-competitive type of inhibition. Ackermann plot indicates that sodium fluoride is a reversible inhibitor of seminal plasma acid phosphatase.
What kind of inhibitor is acid phosphatase?
Fluoride and phosphate are well-known inhibitors of acid phosphatase. Also in our study, they acted as inhibitors for enzyme activity.
What type of inhibitor is nah2po4?
The results show that Na2HPO4, Na2HAsO4 and Na2WO4 are competitive inhibitors, L-phenylalanine is an uncompetitive inhibitor, while L-cysteine is a mixed-type inhibitor.
