What does CRE do in mice?
What does CRE do in mice?
The Cre/lox system is one of the most powerful and versatile tools developed for mouse genetics. It gives mouse researchers sophisticated control over the location and timing of gene expression. Cre/lox is usually used to make knockout alleles, but it can also be used to activate gene expression.
Are mice heterozygous?
The chimera mice are crossed with a normal wildtype mouse (grey). This produces offspring that are either white and heterozygous for the knocked out gene or grey and wildtype. White heterozygous mice can subsequently be crossed to produce mice that are homozygous for the knocked out gene.
Who invented Cre lox?
Nat L. Sternberg
The origins of this tool date to more than 30 years ago when Nat L. Sternberg discovered the recombinase, Cre, and its specific locus of crossover, lox, while studying the maintenance of bacteriophage P1 as a stable plasmid.
What Cre means?
CRE stands for carbapenem-resistant Enterobacterales. Enterobacterales are an order of germs, specifically bacteria. Many different types of Enterobacterales can develop resistance, including Klebsiella pneumoniae and Escherichia coli (E. coli).
Does Cre need to be homozygous?
You should not need a homozygous Cre transgene for efficient recombination. Expression levels from a hemizygous transgene should be enough to see recombination. Randomly integrated transgenes can lead to surprising phenotypes when they are bred to homozygosity.
How do you make heterozygous CRE transgenic mice?
To generate mice that are heterozygous for a loxP -flanked allele and hemizygous/heterozygous for the cre transgene, mate a homozygous loxP -flanked mouse of interest to a cre transgenic mouse strain (see Figure 1 below). Approximately 50% of the offspring will be heterozygous for the loxP allele and hemizygous/heterozygous for the cre transgene.
How to breed Heterozygous loxP mice with Cre-Lox mice?
Again using the same Cre-lox example: 1) Breed your homozygous loxP mice (most will be homs) to your cre transgenic strain, which will generate mice that are heterozygous loxP , hemizygous cre .
What is the most efficient breeding scheme for Cre/Lox tissue-specific knockouts?
Here is the most efficient breeding scheme for generating Cre/ lox tissue-specific or inducible knockouts. To generate mice that are heterozygous for a loxP -flanked allele and hemizygous/heterozygous for the cre transgene, mate a homozygous loxP -flanked mouse of interest to a cre transgenic mouse strain (see Figure 1 below).
How to cross cross breed Cre-Lox with CRE transgenic strain?
Again using the same Cre-lox example: 1 Breed your homozygous loxP mice (most will be homs) to your cre transgenic strain, which will generate mice that are… 2 Cross the heterozygous loxP, hemizygous cre mice back to the homozygous loxP, which will generate some homozygous… More
https://www.youtube.com/watch?v=L3ZBM-GGimI
