What does DMEM medium contain?
What does DMEM medium contain?
DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of glucose and was originally used to culture embryonic mouse cells.
What is DMEM used for?
DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
What is DMEM 10?
Dulbecco’s modified Eagle’s medium (DMEM; GIBCO/Invitrogen) 10% normal calf serum. 1% penicillin/streptomycin (Cellgro) 1% L-glutamine (Cellgro) Filter-sterilize through a 0.22-μm filter.
How can DMEM make blood sugar high?
-For 1 lt 50 mM high glucose DMEM, you need 44.8 ml of 1 M glucose and 955.2 ml of 5.5 mM DMEM, if you wanna make a solution from a liquid glucose concentration. – Or else simply add 8 g of D-glucose into the 1 L of low glucose DMEM.
What does dmem/f12 mean?
DMEM/F12 Complete Medium contains a 1:1 mixture of DMEM medium and Ham′s F12 medium, supplemented with 2mM L-Glut and 10% U.S. origin FBS. DMEM/F12 Complete Medium is a convenient sterile solution for culture of primary mammalian cells as well as a variety of normal and transformed cells
What is the difference between the dmem/f12 and embryomax DMEM with L-glutamine?
The DMEM/F12, with HEPES, L-Glutamine is available in a 500 mL format & has been optimized & validated for Stem cell culture. The EmbryoMax DMEM/F12, with L-Glutamine, without HEPES is available in a 500 mL format and may be used for routine mouse embryonic stem cell culture applications.
What is dmemf12 complete media?
Advanced DMEMF12 basal media used in low-serum or serum-free media formulations including stem cell and 3D organoid cell cultures. DMEM/F12 Complete Medium contains a 1:1 mixture of DMEM medium and Ham′s F12 medium, supplemented with 2mM L-Glut and 10% U.S. origin FBS.
What types of cells can be cultured in DMEM/F-12?
Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
