What happens if the spot line and positions are marked on the plate with a ballpoint pen when preparing a TLC plate for development?

What happens if the spot line and positions are marked on the plate with a ballpoint pen when preparing a TLC plate for development?

What happens if the spot line and positions are marked on the plate with a ballpoint pen when preparing a TLC plate for development? Ballpoint pen ink consists of organic compounds that will move with the development solvent and cause additional spots to appear on the plate.

What happens if a solvent is too polar?

If a development solvent of too high a polarity is used, all components in the mixture will move along with the solvent and no separation will be observed (Rf’s will be too large). If the solvent is of too low a polarity the components will not move enough, and again separation will not occur (Rf’s will be too small).

Which of the following solvents would be the best to separate a mixture containing benzoic acid 2 4 Dinitrobenzoic acid and 2 4 6 Trinitrobenzoic acid by TLC?

Which of the following solvents would be the best to separate a mixture containing benzoic acid, 2,4-dinitrobenzoic acid, and 2,4,6-trinitrobenzoic acid by TLC? Acetone (polar)should work well because the mixture is highly polar.

What is TLC plate?

Thin layer chromatography (TLC) is an affinity-based method used to separate compounds in a mixture. In TLC, the stationary phase is a thin adsorbent material layer, usually silica gel or aluminum oxide, coated onto an inert plate surface, typically glass, plastic, or aluminum.

Which of the following solvents would be the best to separate a mixture containing 2 phenylethanol and acetophenone?

All three given compounds benzoic acid, 2,4 -dinitrobenzoic acid and 2,4,6 -trinitrobenzoic acid are carboxylic acids. They are highly polar compounds. Therefore, acetone would be the best choice of solvent because it is a highly polar solvent.

What would happen if the spotting line was below the solvent line in the chamber group of answer choices?

If the solvent level in the developing jar is deeper than the origin (spotting line) of the TLC plate, the solvent will dissolve the compounds into the solvent reservoir instead of allowing them to move up the plate by capillary action. Thus, you will not see spots after the plate is developed.

Is acetophenone more polar than 2 Phenylethanol?

2 -phenylethanol is an alcohol. Acetophenone is a ketone. When comparing the two structures one can observe that 2 -phenylethanol is more polar than acetophenone. Therefore, one need a moderately polar solvent to effectively separate these two components in a mixture.

Is benzoic acid polar?

The primary reason benzoic acid dissolves only slightly or poorly in cold water is that, because of a polar carboxylic group, the bulk amount of the benzoic acid molecule is non-polar. It is only the carboxylic group that is polar.

How does TLC work polarity?

The eluting power of solvents increases with polarity. Therefore, low polarity compounds can be eluted with low polarity solvents, while higher polarity compounds require solvents of higher polarity. The stronger a compound is bound to the adsorbent , the slower it moves up the TLC plate.

What is the meaning of 2-phenylethanol?

Phenylethyl alcohol is an antimicrobial, antiseptic, and disinfectant that is used also as an aromatic essence and preservative in pharmaceutics and perfumery. 2-Phenylethanol is found in almond. 2-Phenylethanol is a flavouring ingredient. 2-Phenylethanol is a component of ylang-ylang oil.

What is 2phenylethanol used for?

2-phenylethanol is a primary alcohol that is ethanol substituted by a phenyl group at position 2. It has a role as a fragrance, a Saccharomyces cerevisiae metabolite, a plant metabolite, an Aspergillus metabolite and a plant growth retardant. It is a primary alcohol and a member of benzenes. PubChem.

What is the half life of 2-phenylethanol in air?

Vapor-phase 2-phenylethanol is degraded in the atmosphere by reaction with photochemically-produced hydroxyl radicals(SRC); the half-life for this reaction in air is estimated to be 1.6 days(1,SRC). 2-Phenylethanol solubility in water is 16,000 mg/L(4), and therefore it may be washed out of the atmosphere by rain(SRC).

Does 2-phenylethanol have a bioconcentration potential in fish and aquatic organisms?

A BCF of 6 was estimated for 2-phenylethanol (SRC), using its log Kow of 1.36(1,SRC) and a recommended regression-derived equation(2). According to a recommended classification scheme(3), this BCF value suggests that 2-phenylethanol has a low potential for bioconcentration in fish and aquatic organisms(SRC).