What is a DAPI filter?
What is a DAPI filter?
The Nikon dual excitation band DAPI-FITC filter set is designed for the simultaneous detection of the popular fluorophores DAPI and FITC (fluorescein isothiocyanate) when applied in combination, and incorporates an excitation filter with narrow bandpass windows in the violet (400-418 nanometers) and the blue (478-495 …
What is Tritc filter?
Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.
What is Cy3 used for?
The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications. It is also the basis for the classic lipophilic tracer DiI and its variants.
What color is DAPI?
blue
DAPI (4′,6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits ~20-fold enhancement of fluorescence upon binding to AT regions of dsDNA.
What color is DAPI as fluorescence?
DAPI (4′,6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits ~20-fold enhancement of fluorescence upon binding to AT regions of dsDNA. It is excited by the violet (405 nm) laser line and is commonly used as a nuclear counterstain in fluorescence microscopy, flow cytometry, and chromosome staining.
Does DAPI interfere with PCR?
3,3′-Diaminobenzidine staining interferes with PCR-based … These results suggest that the observed reduction in qPCR signal is due to a direct effect on the DNA template derived from DAB-stained tissue, rather than inhibition of the polymerase or signal detection.
What is the Nikon dual excitation band DAPI-FITC filter?
The Nikon dual excitation band DAPI-FITC filter set is designed for the simultaneous detection of the popular fluorophores DAPI and FITC (fluorescein isothiocyanate) when applied in combination, and incorporates an excitation filter with narrow bandpass windows in the violet (400-418 nanometers) and the blue (478-495 nanometers) spectral regions.
What is the wavelength of a DAPI-FITC filter?
Dual Excitation Filter Block DAPI-FITC Specifications Excitation Filter Wavelengths: 400-418 nanometers (bandpass, 409 CWL) and 478-495 nanometers (bandpass, 487 CWL) Polychromatic Mirror Wavelengths: 435-470 nanometers (bandpass) and 505-570 nanometers (bandpass)
Why choose Nikon fluorescence filter cubes?
High-performance/high-quality fluorescence filter cubes for research that demands precise signal detection. Nikon offers a wide range of fluorescence filter cubes with high fluorescence acquisition efficiency to support imaging of a large variety of fluorophores and fluorescent proteins.
Do et filters provide zero pixel-shift image registration?
All ET filters sets provide zero-pixel-shift image registration* between filter sets, at no additional cost. *It is important to note that filters are only one factor in achieving perfect image registration in imaging systems. All other components and devices must also be in register.
