What is a DNA ladder mix?
What is a DNA ladder mix?
Thermo Scientific MassRuler DNA Ladder Mix is specially designed for accurate quantification and sizing of DNA fragments by agarose gel electrophoresis. The ladders are mixtures of chromatography-purified, individual DNA fragments.
What DNA ladder should I use?
The 10bp or 50bp DNA ladder is ideally recommended for 3% gel (restriction digestion) and PAGE. PAGE can separate smaller DNA fragments sharply.
How much ladder should I add?
For a standard electrophoresis system, we recommend loading 0.5 µg (20 µl) of the Fast DNA Ladder on the agarose gel. For a fast electrophoresis system (5 to 30 minutes separation), follow the system’s manufacturer recommendations: 5 to 20 µl load.
Why do gels smile?
The “smile” effect where the center lanes run faster than the outside lanes is common with many gel boxes. One way to reduce that is to run the gel at a lower voltage for a longer time. The “curling” of individual bands is usually a result of overloading the wells (too much DNA).
What is generuler 1 KB plus DNA ladder?
Thermo Scientific GeneRuler 1 kb Plus DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 75 bp to 20,000 bp on agarose gels. The DNA ladder consists of 15 DNA fragments and is provided with 6X TriTrack DNA Loading Dye.
What is Invitrogen 1 KB plus DNA ladder?
Description Invitrogen 1 Kb Plus DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 15,000 bp. 1 Kb Plus DNA Ladder consists of 18 individual chromatography-purified DNA fragments and has a reference band at 1,500 bp for easy orientation.
What is the shelf life of purple 1 KB plus DNA ladder?
Quick-Load Purple 1 kb Plus DNA Ladder is stable for at least 6 months at 25°C. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing. If not well mixed, the load may float out of the wells.
How many DNA fragments are in the DNA ladder?
The DNA ladder consists of 15 DNA fragments and is provided with 6X TriTrack DNA Loading Dye. This double-stranded DNA ladder is compatible with 1% standard and precast agarose slab gels and can be visualized after ethidium bromide or SYBR Safe staining. A uniform intensity of bands and bright reference bands allow easy sizing and orientation.
