What is a hydrodynamic injection?
What is a hydrodynamic injection?
The hydrodynamic tail vein injection is a technique that is used to deliver nucleic acids into live mice. Delivery through this method results in the in vivo transfection of foreign DNA primarily in the liver.
What is hydrodynamic gene delivery?
Hydrodynamic gene delivery uses a hydrodynamic force generated by a pressurized injection of a large volume of DNA solution into the blood vessel so as to permeabilize the capillary endothelium and generate “pores” in the plasma membrane of the surrounding parenchyma cells, through which DNA or other macromolecules of …
How does hydrodynamic sample injection work in capillary electrophoresis?
Hydrodynamic injection is accomplished by the application of a pressure difference between the two ends of a capillary. The amount of sample injected can be calculated by the Poiseuille equation.
How do you inject mouse tail veins?
Insert the needle (small gauge, 27-30 for mice and 25-27 for rats), bevel up, into the vein towards the direction of the head. Keep the needle and syringe parallel to the tail. Aspiration is not advised as it may cause the vein to collapse, but a flash of blood in the hub of the needle may be seen when first placed.
How do you repress the electroosmotic flow?
In aqueous capillary electrophoresis the electroosmotic flow (EOF) can be strongly suppressed or eliminated by coating the capillary surface silanols either by buffer additive adsorption or chemical modification.
What causes electroosmotic flow?
Electroosmotic flow is caused by the Coulomb force induced by an electric field on net mobile electric charge in a solution. The resulting flow is termed electroosmotic flow.
What is IV injection mice?
Intravenous (IV) administration in mice is predominantly performed via the lateral tail veins. The technique requires adequate training before it can be used safely and routinely.
How many veins are in a mouse tail?
The mouse has two lateral tail veins and a tail artery located on the ventrum of the tail. A greater volume of blood may be collected from the artery, but care must be taken to ensure adequate hemostasis, and therefore should only be used if larger amounts are needed.
How can electroosmotic flow be suppressed?
Electroosmotic flow can be reduced by coating the capillary with a material that suppresses ionization of the silanol groups, such as polyacrylamide or methylcellulose.
How can electroosmotic flow be reduced?
What is electroosmotic flow EOF and why does it occur?
