What is a spectrophotometer cuvette?
What is a spectrophotometer cuvette?
Cuvettes are designed to hold samples for spectroscopic measurement, where a beam of light is passed through the sample within the cuvette to measure the absorbance, transmittance, fluorescence intensity, fluorescence polarization, or fluorescence lifetime of the sample.
Which is the most commonly used cuvette in spectrophotometry?
The most common type of cuvette is square, with external dimensions of 12.5 x 12.5 mm. This format accommodates sample volumes from the microliter range (ultra-micro cuvettes) to the milliliter range (macro cuvettes) (figure 1).
What are disposable cuvettes?
A cuvette is a piece of laboratory equipment that is intended to hold samples for spectroscopic analysis. Plastic cuvettes are used in the visible range for colorimetric assays and the UV range for DNA, RNA, and protein analysis. …
What is minimum cuvette volume?
A standard cuvette typically requires a measurement volume between 50 ml and 2 ml. A microvolume adapter requires samples in a single microliter range, typically between 2 ml and 10 ml.
Which is the most sensitive detector for spectrophotometer?
Photomultipliers
Photomultipliers have high sensitivity for UV and visible radiation and have fast response times. However, they are susceptible to damage when exposed to high intensity light. Photomultiplier tube is inherently more sensitive than the photo tube.
How does a dirty cuvette affect absorbance?
On a spectrophotometer which measures how much light is absorbed, it is safe to say that less light will reach the sample in a dirty cuvette. Therefore, the machine will interpret this as more light being absorbed. So, in other words, if the cuvette is dirty, the readings will be off.
What type of plastic are cuvettes made of?
Cuvettes are commonly made from different transparent materials such as optical glass, quartz or transparent plastic. At first sight all such materials appear to be perfectly transparent and fit for all types of absorbance studies.
What are the different types of cuvette cuvettes?
The four most popular cuvette materials are listed below:
- Optical Glass or Pyrex Glass.
- UV Quartz.
- IR Quartz.
- Sapphire.
What is cuvette volume?
Cuvette volume is the maximum amount of sample that a cuvette can safely hold. The most common capacity is 3.5 mL for a standard 10 mm cell, but how do we figure it out?
How does cuvette size affect absorbance?
The absorbance is directly proportional to the concentration (c) of the solution of the sample used in the experiment. The absorbance is directly proportional to the length of the light path (l), which is equal to the width of the cuvette.
Why plastibrand cuvettes?
Plastibrand cuvettes are delivered grouped by mold cavity number to ensure lowest variation of extinction coefficient. Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
What type of cuvettes are available for kinetic measurement?
Macro or semi-micro cuvettes available in PS and PMMA. Ideal for kinetic measements. Windows are recessed to protect from scratches, arrow mark indicates optical path orientation. Plastibrand cuvettes are delivered grouped by mold cavity number to ensure lowest variation of extinction coefficient.
What type of cuvets are used in Fisher brand spectrophotometer?
Description. Fisherbrand™ Disposable Cuvettes are available in choice of polystyrene or methacrylate. Select polystyrene cuvets for assays in visible spectral range (340 to 750nm); for accuracy throughout UV/Vis range (285 to 750nm), choose cuvets made from a superior grade of methacrylate.
Which type of cuvettes are the least expensive?
Polystyrene (PS) cuvettes are the least expensive, however they have the lowest chemical resistance, and are only suitable for visible light applications, with a lower wavelength limit of around 340nm.
