What is Alamar Blue?
What is Alamar Blue?
alamarBlue Cell Viability Reagent is an indigo-colored, non-toxic reagent that detects metabolically active cells and is used for the quantitative analysis of cell viability and proliferation.
What is Alamar Blue test?
The alamarBlue® Assay is designed to measure quantitatively the proliferation of various human and animal cell lines, bacteria and fungi. The bioassay may also be used to establish relative cytotoxicity of agents within various chemical classes (3).
How do you make Blue Alamar?
All Answers (1)
- Dissolve alamar blue in PBS (pH 7.4) to 0.15 mg/ml.
- Filter-sterilize the resazurin solution through a 0.2 μm filter into a sterile, light protected container.
- Store the resazurin solution protected from light at 4°C for frequent use or at -20°C for long term storage.
How is Alamar blue calculated?
O1 = molar extinction coefficient (E) of oxidized alamarBlue (blue) at 570 nm* O2 = E of oxidized alamarBlue at 600 nm* A1 = absorbance of test wells at 570 nm. A2 = absorbance of test wells at 600 nm….62%
| Wavelength | Reduced (R) | Oxidized (O) |
|---|---|---|
| 540 nm | 104395 | 47619 |
| 570 nm | 155677 | 80586 |
| 600 nm | 14652 | 117216 |
| 630 nm | 5494 | 34798 |
What does Trypan Blue do?
Trypan blue is a cell impermeant stain used to estimate the number of dead cells in a viable population. Its utility is based on the fact that it is a charged dye and does not enter cells unless the membrane is compromised.
How does resazurin reduction assay work?
The resazurin assay protocol is based on the reduction of oxidized non-fluorescent blue resazurin to a red fluorescent dye (resorufin) by the mitochondrial respiratory chain in live cells. The amount of resorufin produced is directly proportional to the number of living cells.
Why do dead cells stain blue?
Because live cells have an intact cell membrane, trypan blue cannot penetrate the cell membrane of live cells and enter the cytoplasm. In a dead cell, trypan blue passes through the porous cell membrane and enters the cytoplasm. Under light microscopy analysis, only dead cells have a blue color.
How do you perform LDH?
You’ll have blood drawn through a needle inserted into a vein in your arm. For LDH tests of the cerebrospinal fluid, you’ll need a lumbar puncture (also called a spinal tap). You’ll have a thin needle inserted into your lower back.
First introduced under the Alamar Blue trademark, the dye has been incorporated in commercial assay kits under different names, such as Vision Blue. Visualization of resazurin relies on the activity of cellular dehydrogenases in the presence of NADH/NADPH.
Is alamarblue a reliable reagent?
This is a trusted and established reagent which has been available since 1993. alamarBlue is a useful non-toxic alternative to the commonly used MTT cell viability assay (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide) ( O’Brien et al. 2000 ).
What is therthermo scientific alamarblue?
Thermo Scientific™ alamarBlue provides unmatched sensitivity and versatility, allowing three assays to be performed with just one dye, including cell growth determination, in vitro cytotoxicity and cytokine assays. alamarBlue, a resazurin-based product with extended incubation time, is stable for up to 5 days.
What is the active ingredient in alamarblue?
Resazurin, the active ingredient of alamarBlue reagent, is a non-toxic, cell-permeable compound that is blue in color and virtually non-fluorescent. Upon entering living cells, resazurin is reduced to resorufin, a compound that is red in color and highly fluorescent.
