What is an EAE model?

What is an EAE model?

Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis. This model is produced by administering a myelin basic protein peptide (MBP) fragment that induces an autoimmune response directed to the myelin sheath surrounding motor neurons.

Is EAE a model of MS?

Experimental autoimmune encephalomyelitis (EAE) is the most commonly used experimental model for the human inflammatory demyelinating disease, multiple sclerosis (MS).

How do you induce an EAE on a mouse?

EAE is induced in C57BL/6 mice1 by immunization with an emulsion of MOG35-55 or MOG1-125 in complete Freund’s adjuvant (CFA), followed by administration of pertussis toxin in PBS, first on the day of immunization and then again the following day.

What is MOG EAE?

Abstract. Myelin oligodendrocyte glycoprotein (MOG) is a transmembrane glycoprotein expressed on the surface of central nervous system (CNS) myelin membranes, which has been shown to induce experimental autoimmune encephalomyelitis (EAE) in rodents.

Can u reject EAE?

Applicants will receive notification of the release of the Poly EAE posting results via email. Successful applicants are to confirm the acceptance of their offers through the online portal. A non-response will be deemed as a rejection of the offer.

Can mice get multiple sclerosis?

In the SJL/J mouse, both active induction and adoptive transfer of disease typically take a relapsing–remitting form, while the C57BL/6 mouse displays chronic–progressive disease following active or passive EAE induction. TMEV-IDD has been defined as a mouse model for human multiple sclerosis ( 29, 30 ).

What happens when demyelination occurs?

Demyelination is loss of myelin, a type of fatty tissue that surrounds and protects nerves throughout the body. This condition causes neurological deficits, such as vision changes, weakness, altered sensation, and behavioral or cognitive (thinking) problems.

What is EAE mouse?

EAE by Active Immunization In the active immunization method, the mouse is immunized using neuro antigens, such as myelin antigens or spinal cord homogenate, which causes development of activated myelin-specific T cells, trafficking of immune cells into the CNS, and direct damage and inflammation in the CNS.

What are SJL mice?

SJL mice display a very high incidence of reticulum cell sarcomas resembling Hodgkin’s disease by approximately one year of age. This strain is also characterized by extreme aggression in males and its susceptibility to experimental autoimmune encephalomyelitis (EAE) for multiple sclerosis research.

What is mog35 55 peptide?

MOG (35-55) is a myelin oligodendrocyte glycoprotein (MOG) 35-55 is a minor component of CNS myelin. Produces a relapsing-remitting neurological disease with extensive plaque-like demyelination, common to the manifestations of multiple sclerosis. Induces strong T and B cell responses and is highly encephalitogenic.

How does MOG cause EAE?

The pathophysiology of EAE is based on the reaction of the immune system against brain-specific antigens. This reaction induces inflammation and destruction of the antigen carrying structures, resulting in neurological and pathological features comparable which those observed in MS patients.

What should I write for EAE?

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Is there a standard scoring scale for EAE disease severity?

While standard scoring of EAE disease severity on a 0–5 numerical scale is commonly used by scientists all over the world, there is a likelihood of operator/assessor-dependent variability as the clinical scoring between different researchers is highly subjective.

What is a 0 5 on the EAE scale?

These defects are quantified using a standard EAE scoring system on a 0–5 disease severity scale: 0, no disease; 1, loss of tail tone; 2, hind limb weakness; 3, hind limb paralysis; 4, hind limb paralysis and forelimb paralysis or weakness; and 5, moribund/death 10.

How can we improve reliability of EAE scoring?

Reliable EAE scoring requires skill which comes after considerable experience. To avoid unconscious bias in scoring, we strongly recommend that mice should be scored blind, by a person unaware of which mice have received which treatment. We recommend the following scoring guidelines for mice during onset and peak of EAE:

What is the normal range for EAE?

Typically, EAE is scored on scale 0 to 5. Most researchers also give mice “in-between” scores (i.e. 0.5, 1.5, 2.5, 3.5) when the clinical picture lies between two defined scores. The scoring method differs slightly depending on the stage of disease (onset/peak vs. recovery), for each individual mouse.