What is bicistronic expression vector?
What is bicistronic expression vector?
Bicistronic expression vector for the simultaneous translation of two genes of interest from the same mRNA transcript. Each gene should be inserted into one of two MCSs located on either side of the ECMV internal ribosomal entry site (IRES). The entire cassette is driven by the CMV promoter.
What is a Bicistronic gene?
As the name suggests, bicistronic plasmids contain two distinct genes of interest within one vector. The vector transports the genes together into the cells, which means that every cell with one gene also has the other.
What is a Bicistronic plasmid?
Introducing the bicistronic plasmid As the name suggests, bicistronic plasmids contain two distinct genes of interest within one vector. The vector transports the genes together into the cells, which means that every cell with one gene also has the other.
What is Bicistronic design?
(Mutalik, Endy, Guimaraes, Cambray, Lam, Juul, Tran & Paull, 2013) The Bicistronic design(BCD) consists of two Shine-Dalgarno sequences in its translation element which when combined with indiscriminate gene of interests are known to reliably express within twofold of the relative target expression window.
What is a Bicistronic construct?
What does pires2-zsgreen1 stand for?
IRES: internal ribosome entry sequence; FP1: fluorescent protein (AcGFP1, DsRed-Monomer, or mCherry); FP2: fluorescent protein (mCherry or ZsGreen1); MCS: multiple cloning site. pIRES2-ZsGreen1 is a bicistronic expression vector containing ZsGreen1 as a marker for transfection efficiency.
How can I see the zsgreen1-ires sequence?
IRES-containing bicistronic vector for expressing a gene together with the fluorescent protein ZsGreen1. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer. Your time is valuable! × Please choose an application for opening sequence files.
What is the IRES sequence used for?
The IRES sequence allows the gene of interest and ZsGreen1 to be translated simultaneously from the same mRNA transcript. Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind.
Which lentiviral packaging system to use for zsgreen1- expressing lentiviruses?
Lenti-X 293T Cell Line. Cat. # 632180. Clontech’s lentiviral packaging system (Panel A) and a lentiviral packaging system from a leading competitor (Panel B) were each used to generate viral supernatants from their respective lentiviral system vectors that were engineered to express the ZsGreen1 fluorescent protein.
