What is C11 bodipy?
What is C11 bodipy?
Product Description. C11 BODIPY 581/591 is a lipid-soluble ratiometric fluorescent indicator of lipid oxidation. 1. Upon oxidation, its excitation maximum shifts from 581 to 500 nm and the emission maximum shifts from 591 to 510 nm. C11 BODIPY 581/591 has been used in the quantitation of ferroptosis.
What causes lipid peroxidation?
Lipid peroxidation is a process generated naturally in small amounts in the body, mainly by the effect of several reactive oxygen species (hydroxyl radical, hydrogen peroxide etc.). It can also be generated by the action of several phagocytes.
What is Ferrostatin?
Ferrostatin 1 is a selective inhibitor of erastin induced ferroptosis (EC50 = 60 nM). Specifically inhibits Ras selective lethal compound -induced death, but not cell death induced by other oxidative lethal compounds and apoptosis-inducing agents.
What is peroxidation reaction?
Lipid peroxidation is the chain of reactions of oxidative degradation of lipids. It is the process in which free radicals “steal” electrons from the lipids in cell membranes, resulting in cell damage. This process proceeds by a free radical chain reaction mechanism.
What causes lipolysis?
Lipolysis is triggered by the activation of adenyl cyclase, which converts adenosine triphosphate (ATP) into cyclic adenosine monophosphate (cAMP). Catecholamines, acting via beta-adrenergic receptors (βADRs), stimulate adenyl cyclase but this action is counteracted by activation of alpha-adrenergic receptor (αADR).
Can bodipy be fixed?
We routinely add BODIPY 493/503 from a 1 mg/ml stock to fixed or living cells at a final concentration of 1 μg–10 μg/ml; followed by at least a 1-h incubation at 37°C. The final concentration of BODIPY 493/503 depends on fat droplet size, the larger the fat droplets, the less stain is needed.
What is bodipy used for?
BODIPY dyes have been used extensively in recognition and imaging applications owing for their improved stability toward photobleaching as well as high quantum yield (Φ) and high molar absorption coefficient (ɛ) values.
How can you prevent ferroptosis?
Ferroptosis can be prevented by using iron chelators (e.g., deferoxamine), whereas supplying exogenous iron (e.g., ferric ammonium citrate) enhances ferroptosis.
What is c11-bodipy used for?
Abstract. C11-BODIPY (581/591) is a fluorescent radio-probe for indexing lipid peroxidation and antioxidant efficacy in model membrane systems and living cells, with excellent characteristics: (i) emission in the visible range of the electromagnetic spectrum, with good spectral separation of the nonoxidized (595 nm) and oxidized (520 nm) forms;
What is c11-bodipy fluorescent?
C11-BODIPY 581/591 is a fluorescent fatty acid analogue with fluorescent properties in the red range of the visible spectrum (emission maximum 595 nm), allowing its application in fluorescence microscopy. Upon free radical-induced oxidation, its fluorescent properties shift from red to green.
What is c11-bodipy591/581 staining?
C11-BODIPY591/581 staining was combined with flow cytometry measurements to detect reactive oxygen species (ROS) and oxidative stress damage in living cells and membrane systems. Oxidation of the polyunsaturated butadienyl portion of the dye results in a shift of the fluorescence emission peak from ~590 nm to ~510 nm.
Can the c11-bodipy dye be used to determine lipid oxidation in Chlamydomonas?
Optimization of the C11-BODIPY(581/591) dye for the determination of lipid oxidation in Chlamydomonas reinhardtii by flow cytometry Lipid oxidation is a recognized end point for the study of oxidative stress and is an important parameter to describe the mode of micropollutant action on aquatic microorganisms.
