What is e-cadherin antibody?

What is e-cadherin antibody?

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity.

What is the function of E-cadherin?

E-cadherin is thought to prevent the initial dissociation of epithelial cells from the original tumor mass, and loss of cell-cell adhesion and cell junctions allows cells to invade surrounding tissues and migrate to distant sites.

What cells express E-cadherin?

Our interest was stimulated by studies indicating that E-cadherin is expressed by Langerhans cells which are part of the family of dendritic cells [8, 9].

Is E-cadherin a classical cadherin?

There are about one hundred types of cadherins in vertebrates, and they fall into four groups. Classical cadherins include E-cadherin, N-cadherin, P-cadherin and N-cadherin 2. They all have a similar structure, with five extracellular cadherin repeats, a transmembrane domain, and an intracellular domain.

What does E-cadherin stand for?

Cadherin-1 (not to be confused with the APC/C activator protein CDH1) also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin is a protein that in humans is encoded by the CDH1 gene. Mutations are correlated with gastric, breast, colorectal, thyroid, and ovarian cancers.

Is E-cadherin a tumor suppressor?

E-cadherin is a tumor suppressor protein, and the loss of its expression in association with the epithelial mesenchymal transition (EMT) occurs frequently during tumor metastasis.

What causes loss of E-cadherin?

In human tumors, loss or reduction of E-cadherin expression can be caused by somatic mutations, chromosomal deletions, proteolytic cleavage, and silencing of the CDH1 promoter ( 15– 18).

Is E-cadherin a protein?

The transmembrane protein E-cadherin is the major protein that mediates homophilic adhesion between neighbouring cells and is, therefore, one of the critical components for epithelial integrity.

Where is E-cadherin located?

E-cadherin is one of the most important molecules in cell-cell adhesion in epithelial tissues. It is localized on the surfaces of epithelial cells in regions of cell-cell contact known as adherens junctions [3].

What is the E-cadherin antigen affinity-purified polyclonal antibody?

E-Cadherin was detected in immersion fixed mouse intestinal organoids using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature.

How is E-cadherin detected in human epidermoid carcinoma cells?

E-Cadherin was detected in immersion fixed human epidermoid carcinoma cells using 10 µg/mL Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) for 3 hours at room temperature.

Is there E-cadherin in D3 mouse embryonic stem cell line?

E‑Cadherin in D3 Mouse Embryonic Stem Cell Line. E-Cadherin was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature.

How do you detect E-cadherin in human stomach?

E-Cadherin was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004 ).