What is Fontana Masson stain used for?
What is Fontana Masson stain used for?
The Fontana-Masson Stain is intended for use in the histological visualization of Argentaffin cells and Melanin in paraffin or frozen sections. In addition, the Fontana-Masson stain has been reported to be useful in identifying Capsule-Deficient Cryptococcus Neoformans and typical Cryptococcus Neoformans.
What stain is used for Cryptococcus neoformans?
For identification in tissue, mucicarmine stain provides specific staining of polysaccharide cell wall in C. neoformans.
What is mucicarmine stain used for?
Mucicarmine stain is intended for the staining of mucin. Mucin is a secretion produced by a variety of epithelial cells and connective tissue cells. In certain intestinal inflammations or carcinomas, an excess of mucin is secreted by the epithelial cells.
Is Cryptococcus an Ascomycete?
Cryptococcus neoformans is a basidiomycete fungus that causes cryptococcosis worldwide in both immunocompromised and healthy individuals. As a basidiomycete, it diverged from other common pathogenic or model ascomycete fungi more than 500 million years ago.
Is oil a red?
Oil Red O is a fat-soluble dye that stains neutral triglycerides and lipids. It cannot be used with formaldehyde-fixed paraffin embedded sections as the alcohols used remove most lipids….Staining Interpretation.
| Fat Cells | Red |
|---|---|
| Neutral Fat | Red |
| Nuclei | Blue |
What kind of stain is most commonly used to visualize the capsule of Cryptococcus?
To visualize the capsule perimeter, we used India ink staining, which is excluded by the capsule, and the capsule can be exhibited by a halo around the cells.
Does Cryptococcus stain with PAS?
Similar to other yeast-form fungi, cryptococcal species stain with GMS, PAS and A-B. They may be differentiated from other pathogenic yeasts using MUC, which stains the mucoid capsule, and F-M stain, which reveals melanin present in the cell wall of the organism.
What does Cryptococcus neoformans look like?
Cryptococcus neoformans is a round or oval yeast (4–6 μm in diameter), surrounded by a capsule that can be up to 30 μm thick. The organism grows readily on fungal or bacterial culture media and is usually detectable within 1 week after inoculation, although in some circumstances up to 4 weeks are required for growth.
What is the Cryptococcus?
Cryptococcus is an invasive fungus, transmitted through the inhalation of spores and causes cryptococcosis, an infection commonly associated with immunosuppressive individuals. Patients present with fever, headache, malaise, photophobia and neck stiffness as cryptococcal meningitis sets in.
