What is Jet PEI?
What is Jet PEI?
jetPEI® transfection reagent is a linear polyethylenimine derivative, free of components of animal origin, providing highly effective and reproducible gene delivery to adherent and suspension cells.
What is vivo transfection?
TurboFect in vivo is a sterile solution of a cationic polymer in water. The polymer forms compact, stable, positively charged complexes with DNA. These complexes protect DNA from degradation and facilitate gene delivery in vivo.
What is PEI pro?
PEIpro® is a ready to use 1 mg/ml optimised linear polyethylenimine (PEI) reagent for the production of virus by Transient Gene Expression (TGE) in suspension-adapted mammalian cell lines cultivated in shaker flasks, platform shakers or stirred tank bioreactors.
What is PEI transfection?
DNA can be introduced into a host cell by transfection with polyethylenimine (PEI), a stable cationic polymer (Boussif et al., 1995). PEI condenses DNA into positively charged particles that bind to anionic cell surfaces. Our laboratory uses PEI over other cell transfection reagents because of its low cost.
What is lipofectamine transfection?
Lipofectamine or Lipofectamine 2000 is a common transfection reagent, produced and sold by Invitrogen, used in molecular and cellular biology. It is used to increase the transfection efficiency of RNA (including mRNA and siRNA) or plasmid DNA into in vitro cell cultures by lipofection.
Can transfection be done in vivo?
in vivo transfection reagents are the most powerful alternative to viral vectors for nucleic acid delivery. in vivo-jetRNA® is a transfection reagent specifically developed to deliver mRNA in vivo. This reagent can be used to target multiple organs, by using systemic or local injection routes, in various animal models.
Is vitro a transfection?
In vitro transfection refers to the delivery of cargo molecules (such as nucleic acids – DNA or RNA) into cultured cells (usually cancer cell lines). In vivo transfection refers to delivery of cargo molecules (such as therapeutic siRNA, plasmid DNA, small protein, etc) to the target tissue.
How do you make polyethyleneimine?
Preparation of PEI Stocks
- Dissolve 100 mg in 100 mL sterile ddH2O.
- Stir while slowly adding HCl to pH 7.0.
- Mix for 10 minutes and then recheck pH.
- Filter sterilize through 0.22 um filter.
- Aliquot 500 uL to 1000 uL and store in -80C.
- Thawed solution can be stored at 4C for up to 2 months, label a tube when thawed.
What is transient transfection?
In transient transfection, the introduced nucleic acid exists in the cell only for a limited period of time and is not integrated into the genome. Transient transfection is most efficient when supercoiled plasmid DNA is used, presumably due to its more efficient uptake by the cell.
Can Lipofectamine be used in vivo?
As a prelude to direct in vivo gene transfer studies, we have compared the ability of two commercially available cationic lipids (Lipofectamine and DOSPER) to transfect C2C12 cells, a mouse muscle cell line in vitro. In addition, formu- lations suitable for in vivo injection were determined.
What is the difference between electroporation and Nucleofection?
Based on the physical method of electroporation, nucleofection uses a combination of electrical parameters, generated by a device called Nucleofector, with cell-type specific reagents. In contrast, other commonly used non-viral transfection methods rely on cell division for the transfer of DNA into the nucleus.
What is the delivery information for in vivo-jetPEI®?
Moreover, absence of endotoxin is verified in every lot of in vivo-jetPEI®. Delivery information: 10% glucose solution is included. 0.1 ml of in vivo-jetPEI® delivery reagent is sufficient to perform up to 20 intravenous injections in mouse with 50 µg of DNA.
What preclinical transfection reagent is equivalent to in vivo -jetPEI®?
For in vivo preclinical studies, Polyplus-transfection ® supplies a ready-to-use preclinical transfection reagent in vivo-jetPEI®-PC equivalent in potency to in vivo -jetPEI®: (Table 1)
Can I use in vivo -jetPEI ® nanoparticles in mice?
Fig 2: in vivo -jetPEI ® protocol in mice. This two-step protocol is compatible with direct injection of in vivo -jetPEI ® /nucleic acid nanoparticles though any systemic or local administration routes. Our delivery experts are available to adapt your protocol to your animal model and send you the relevant literature.
Does in vivo -jetPEI ® -PC mediate systemic delivery of nucleic acid?
Following in vivo -jetPEI ® -PC, alike in vivo -jetPEI ® mediated systemic delivery of nucleic acid, there is no induction of major pro-inflammatory cytokines (Figure 1) and no increase in sera levels of hepatic enzymes (Figure 2) ( Bonnet et al., 2008 ).
