What is maltose standard curve?
What is maltose standard curve?
The standard curve for maltose is usually constructed using 3, 5-Dinitro salicylic acid (DNS) as the reagent. Maltose reduces the pale yellow coloured alkaline 3,5-Dinitro salicylic acid (DNS) to the orange- red coloured, 3 amino,5 nitro salicylic acid. The plot thus obtained is called a standard maltose curve.
What is the structure of maltose?
C12H22O11Maltose / Formula
How do you make standard maltose solution?
Maltose stock solution: 5 mM/ml. Prepare by dissolving 180 mg maltose in 100 ml distilled water and incubate at 37°C for 10 minutes prior to assay. Enzyme (§-amylase) solution. Dilute to a concentration of 1-10 µg/ml.
How is maltose measured?
The maltase assay utilizes a spectrophotometer and glucose standard curve to measure the amount of glucose released from maltose within a given time period. This value will be your measure of maltase activity. In order to assay maltase activity, you will need a solution containing the enzyme.
What is the Colour of DNS reagent?
Yellow needles
3,5-Dinitrosalicylic acid
| Names | |
|---|---|
| Molar mass | 228.116 g·mol−1 |
| Appearance | Yellow needles or plates |
| Melting point | 182 °C (360 °F; 455 K) |
| Solubility in water | Soluble |
What is the Iupac name of maltose?
2-(hydroxymethyl)-6-[4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyox ane-3,4,5-triolMaltose / IUPAC ID
What is R in standard curve?
The r or r2 values that accompany our calibration curve are measurements of how closely our curve matches the data we have generated. The closer the values are to 1.00, the more accurately our curve represents our detector response. Generally, r values ≥0.995 and r2 values ≥ 0.990 are considered ‘good’.
How do you calculate standard curve?
Standard Curves To calculate the sample concentration based on the standard curve, first you find the concentration for each sample absorbance on the standard curve; then you multiply the concentration by the dilution factor for each sample.
What is maltose concentration?
In order to get a sufficient absorbance difference, the concentration of maltose (inclusive sucrose and D-glucose) in the sample solution used for the assay should range between 0.10-1.0 g/l (measurement at 365 nm) or 0.05-0.5 g/l (measurement at 340, 334 nm).
How to construct a standard curve / graph for maltose?
Constructing a standard curve / graph for maltose helps us to estimate concentration of reducing sugars present in an unknown sample and for determining the activity of amylase enzyme in forthcoming experiments.The standard curve for maltose is usually constructed using 3, 5-Dinitro salicylic acid (DNS) as the reagent.
Is maltose a reducing or reducing sugar?
Maltose is a reducing sugar (those carbohydrates which have a free aldehyde or keto group can reduce Fehling’s and Benedict’s reagents are called reducing sugars). Maltose can be used as a standard for estimating reducing sugar in unknown samples.
How do you measure optical densities of maltose?
A series of solutions containing varying concentrations of maltose are prepared in test tubes and a known quantity of DNS is added to each. These test tubes are then heated on a water bath for few minutes and their optical densities are measured using a colorimeter.
What is maltose made up of?
Maltose is a disaccharide made up of two subunits of glucose monomers. It is also called malt sugar. It is present in germinating grain, in a smaller amount in corn syrup, and also is a product of the partial hydrolysis of starch. Maltose is considered as an important constituent in making fermented barley which is used to brew beer.
