What is Q FF?
What is Q FF?
HiTrap Q FF is a strong anion exchange chromatography column for small-scale protein purification. Packed with Q Sepharose Fast Flow strong anion exchange resin. Convenient and affordable for fast ion exchange separations, either alone or connected in series. High flow rates and good scale-up potential.
What is AQ Sepharose column?
Q Sepharose Big Beads is a strong anion exchanger designed for industrial applications in the capture step of large volumes of feed or viscous feedstocks. HiTrap Q HP, 1 x 1 ml. HiTrap Q HP is a strong anion exchange chromatography column for high-resolution, small-scale protein purification.
What is Q column?
In an anion exchange column, the packing is positively charged and therefore retains negatively charged molecules by coulombic interaction. The strong anion exchange matrix is quaternary ammonium, and is designated Q. The weak anion exchange matrix is diethylaminoethyl, or DEAE.
What is DEAE column?
SMT DEAE [Di-Ethyl-Amino-Ethyl] column provides a unique chemically attached hydrophilic, weak anion exchange type, functional surface desirable for the separation of many biomolecules such as proteins, nucleotides, oligonucleotides, polynucleotides, high molecular weight RNA’s and plasmid DNA’s.
How do you pack Q Sepharose column?
When packing Sepharose 4 Fast Flow media in INdEX columns to bed heights of 15 and 30 cm, the optimal hydraulic packing pressure is between 0.7 bar and 0.8 bar. The final mechanical compression at the end of the packing procedure should be about 5 mm.
How does a phenyl Sepharose column work?
High Performance Columns Phenyl Sepharose HP is based on a matrix of highly cross-linked agarose beads, which have been derivatized with phenyl groups. These are attached to the agarose matrix via uncharged, chemically stable ether linkages. The result is a truly hydrophobic medium with no ionic properties.
How do you clean Q Sepharose?
you can easily clean Q sepharose resin with 4-5 culun volume of 2M NaCl then followed by 8M Urea or 7M guanidium hydrochloride and excess for distilled water and try to store your resin with at least 20% ethanol for long term storage. You can never fully clean Q sepharose.
How do you clean a Q Sepharose column?
What is QQ Sepharose fast flow?
Q Sepharose Fast Flow is a strong anion exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications. Q Sepharose Fast Flow is part of the Sepharose Fast Flow ion exchange…
What is hihitrap Q Sepharose FF GE Healthcare?
HiTrap Q Sepharose FF GE Healthcare. …Q FF is prepacked with Q Sepharose Fast Flow, and is a strong anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.
What is Sepharose XL used for?
…in the elution pool.Q Sepharose XL is a strong anion exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks. Q Sepharose XL is based on a highly cross-linked, bead formed 6% agarose matrix similar to the well established Q Sepharose Fast Flow media. Dextran… …pool.
What is hiprep Q FF 16/10 used for?
Q FF 16/10 is a strong anion exchanger prepacked with Q Sepharose Fast Flow, ready-to-use for fast, preparative separations of proteins and other biomolecules using ion exchange (IEX) chromatography. HiPrep Q FF are prepacked, ready to use columns for ion exchange chromatography.
