What is SalI restriction enzyme?
What is SalI restriction enzyme?
Description: SalI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the SalI gene from Streptomyces albus G. SalI activity is blocked by CpG methylation. NZYTech’s SalI is an enzyme that acts at 37 °C.
What type of nucleic acid does the restriction endonuclease BamHI cut?
Restriction endonuclease BamHI bound to a non-specific DNA. BamHI (pronounced “Bam H one”) (from Bacillus amyloliquefaciens) is a type II restriction endonuclease, having the capacity for recognizing short sequences (6 bp) of DNA and specifically cleaving them at a target site.
What type of restriction enzyme is HindIII?
Introduction. Endonuclease HindIII is a type II restriction enzyme which recognizes and cleaves the palindromic sequence AAGCTT in the presence of Mg2+.
How do the BamHI and EcoRI restriction enzymes cut?
EcoRI binds DNA from the major groove side, whilst EcoRV approaches DNA from the minor groove side. The BamHI recognition sequence differs by only one base pair in each half site from the EcoRI sequence 5′-GAATTC-3′. Both enzymes cleave the DNA at iden- tical positions, following the 5’G on each strand.
Where does the restriction enzyme BamHI cut?
(1995). BamHI binds at the recognition sequence 5′-GGATCC-3′ , and cleaves these sequences just after the 5′-guanine on each strand. This cleavage results in “sticky ends” which are 4 b.p. long. In its unbound form, BamHI displays a central b sheet, which resides in between a helices .
What is Thermo Scientific FastDigest Sali restriction enzyme?
Thermo Scientific FastDigest SalI restriction enzyme recognizes G^TCGAC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Thermo Scientific FastDigest SalI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
What is Sali (FastDigest Sali)?
Thermo Scientific FastDigest SalI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
What are Fermentas FastDigest enzymes?
Thermo Scientific Fermentas FastDigest enzymes are an advanced line of restriction enzymes for rapid DNA digestion. During our 30 years of restriction enzyme research, we compiled one of the largest collections of restriction enzyme producing bacterial strains in the industry.
What is the FastDigest green buffer and Thermo Scientific fast Digest buffer?
The FastDigest Green Buffer and Thermo Scientific FastDigest Buffer are proprietary digestion buffers which support 100% activity of all FastDigest restriction enzymes. This system allows for double and multiple digestions with any combination of enzymes.
